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Could contaminant induced mutations lead to a genetic diversity overestimation?
Sobral, Olímpia; Marin-Morales, Maria Aparecida; Ribeiro, R.Contaminant driven genetic erosion reported through the inspection of selectable traits can be underestimated using neutral markers. This divergence was previously reported in the aquatic system of an abandoned pyrite mine. The most sensitive genotypes of the microcrustacean cladoceran Daphnia longispina were found to be lacking in the impacted reservoir near the entrance of the metal rich acid mine drainage (A...
In Vitro Development of Parthenogenetic Eggs: A Fast Ecotoxicity Test with Daph...
Sobral, Olímpia; Chastinet, Carla; Nogueira, António; Soares, Amadeu M. V. M.; Gonçalves, Fernando; Ribeiro, RuiIn the present study, the authors investigated and defined development stages of Daphnia magna eggs that could be potential endpoints for sublethal toxicity tests with in vitro parthenogenetic egg cultures. Such an "egg test" could be a cost-effective alternative to the classic 21-day test with D. magna. Three main stages of embryonic development were considered: release of the egg external membrane, release of...
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