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Vanadium and cadmium in vivo effects in cardiac muscle: metal accumulation and ...
Soares, S. S.; Martins, H.; Gutiérrez-Merino, Carlos; Aureliano, M.Several biological studies associate vanadium and cadmium with the production of reactive oxygen species (ROS), leading to lipid peroxidation and antioxidant enzymes alterations. The present study aims to analyse and compare the oxidative stress responses induced by an acute intravenous exposure (1 and 7 days) to a sub-lethal concentration (5 mM) of two vanadium solutions, containing different vanadate noligome...
Vanadate induces necrotic cell death in neonatal rat cardiomyocytes through mit...
Soares, S. S.; Henao, Fernando; Aureliano, M.; Gutiérrez-Merino, CarlosBesides the well-known inotropic effects of vanadium in cardiac muscle, previous studies have shown that vanadate can stimulate cell growth or induce cell death. In this work, we studied the toxicity to neonatal rat ventricular myocytes (cardiomyocytes) of two vanadate solutions containing different oligovanadates distribution, decavanadate (containing decameric vanadate, V10) and metavanadate (containing monom...
Vanadium distribution, lipid peroxidation and oxidative stress markers upon dec...
Soares, S. S.; Martins, H.; Duarte, Rui O.; Moura, José J. G.; Coucelo, Josefina; Gutiérrez-Merino, Carlos; Aureliano, M.The contribution of decameric vanadate species to vanadate toxic effects in cardiac muscle was studied following an intravenous administration of a decavanadate solution (1 mM total vanadium) in Sparus aurata. Although decameric vanadate is unstable in the assay medium, it decomposes with a half-life time of 16 allowing studying its effects not only in vitro but also in vivo. After 1, 6 and 12 h upon decavanada...
Decavanadate induces mitochondrial membrane depolarization and inhibits oxygen ...
Soares, S. S.; Gutiérrez-Merino, Carlos; Aureliano, M.Decavanadate induced rat liver mitochondrial depolarization at very low concentrations, half-depolarization with 39 nM decavanadate, while it was needed a 130-fold higher concentration of monomeric vanadate (5 lM) to induce the same effect. Decavanadate also inhibits mitochondrial repolarization induced by reduced glutathione in vitro, with an inhibition constant of 1 lM, whereas no effect was observed up to 10...
The pathways of cell death in cardiomyocytes induced by vanadate
Aureliano, M.; Soares, S. S.; Henao, Fernando; Gutiérrez-Merino, CarlosAfter 24 hours, cardiac myocytes exposure to 10 μM (LD50) vanadate (meta or decavanadate) an increased (30%) of caspase 3-activation was observed, although not significant. On contrary, a significant decrease (40%) of ATP content, characteristic of necrotic cell death was detected. Furthermore, vanadate treatment increased intracellular Ca2+ level from 60 nM to 240 nM, whereas it decreases mitochondria superoxi...
Contribuição de decavanadato para os efeitos IN VIVO e IN VITRO de vanadato n...
Soares, S. S.Tese dout., Biologia - Fisiologia Animal, Universidade do Algarve, 2007 ; No presente estudo aborda-se a contribuição da espécie decamérica de vanadato nos efeitos tóxicos in vivo e in vitro de vanadato. Após administração intravenosa de decavanadato em Sparus aurata, o vanadato decamérico induz respostas nos marcadores de stresse oxidativo do tecido cardÃaco distintas das promovidas pelo monovanadat...
Decavanadate toxicity effects following in vivo administration
Soares, S. S.; Gutiérrez-Merino, Carlos; Aureliano, M.Very few in vivo animal studies involving vanadium consider the contribution of decavanadate (V10) to vanadium biological effects. Recently, it is been suggested that decameric vanadate may not completely fall apart into other vanadate oligomers before induces changes in cell homeostasis, namely in several stress markers. An acute exposure of different fish species (Halobactrachus didactilus, Lusitanian toadfis...
Vanadate oligomers: in vivo effects in hepatic vanadium accumulation and stress...
Gândara, Ricardo M. C.; Soares, S. S.; Martins, H.; Gutiérrez-Merino, Carlos; Aureliano, M.The formation of vanadate oligomeric species is often disregarded in studies on vanadate effects in biological systems, particularly in vivo, even though they may interact with high affinity with many proteins. We report the effects in fish hepatic tissue of an acute intravenous exposure (12, 24 h and 7 days) to two vanadium(V) solutions, metavanadate and decavanadate, containing different vanadate oligomers ad...
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