Considering IPPC climate change scenarios, it is pertinent to predict situations where coastal ecosystems already impacted with chemical contamination became exposed to an additional stressor under a future scenario of seawater intrusion. Accordingly, the present study aimed at evaluating if a negative association between tolerance to a metal and to saltwater exists among genotypes of a freshwater organism. For...
The present study assesses the degree of genetic structure and the presence of recent genetic bottlenecks in the wild boar population in Portugal. One hundred and ten individuals were sampled after capture during organised legal drive hunts, conducted in 58 municipalities across the continental territory, during the game seasons of 2002/2003 and 2003/2004. Individuals were genetically typed Lit six microsatelli...
The drainage of abandoned mines leads to several ecological problems, particularly the acidification of surface freshwater systems and heavy metal contamination. In order to study the possibility of using the behavioural early warning responses of Portuguese indigenous benthic invertebrates to detect an acute short-term pulse of Acid Mine Drainage (AMD), experiments with the Multispecies Freshwater Biomonitor M...
Steroid compounds have been globally detected in surface waters. The ecological impacts of these biologically active chemicals are largely unknown. Toxicity of testosterone and 4-hydroxyandrostenedione was assessed for the freshwater cladoceran Daphnia magna. Acute toxicity tests showed that 6.20 mg L-1 of testosterone, the highest concentration tested, did not have effect on the daphnids, whereas 4-hydroxyandr...
In this study we investigated the causes of seasonal variation in the home ranges of roe deer reintroduced to the Gardunha Mountains (Portugal). From May 2002 to April 2003, 1 year after the animals had been released, we collected data using radio-tracking techniques for five monitored animals (two males and three females). We found differences in the size of home ranges between seasons, with home ranges larger...
We studied the fish assemblages of thirty one, 2(nd)-4(th) order "least- impacted" streams with a varying degree of low-level management in central Portugal, using a standardised survey to document the river habitat. Channel, banks and riparian landuse, described separately according to principal component scores, were significantly related to altitude, slope and management intervention. Species diversity was l...
In this work, ecotoxicological tests were performed, using the estuarine mysid Mesopodopsis slabberi. After an acclimation period mysids were exposed to different sublethal lead concentrations for 48h. The concentrations tested were 0.005, 0.01, 0.02, 0.04, 0.08, 0.16, 0.32, 0.64, 0.80, 1.00, 1.20 and 1.40 mgL(-1), with seven replicates for each concentration. Mortality and physicochemical parameters were measu...
Microorganisms play a crucial role in decomposition processes and nutrient cycling and, therefore, in soil quality. Soil enzymes have shown sensitivity to contaminants, such as heavy metals, due to their interaction with the specific reaction sites, thereby reducing the formation of the reaction products. In this study, several enzymatic bioassays were applied (dehydrogenase, acid phosphatase, arylsulfatase, ur...
This study was designed to evaluate the suitability of an in situ microalgal bioassay with Pseudokirchneriella subcapitata to be used in freshwater environments. The assay potential was investigated by deploying it in a system impacted by acid mine drainage. Water samples were collected to perform a laboratory assay also. P. subcapitata was viewed to be a good option for the in situ assay because it grew well a...
The effects of carbendazim on substrate induced respiration (SIR), dehydrogenase activity (DHA), phosphatase activity and thymidine incorporation by bacteria were evaluated in an experiment with an open intact Terrestrial Model Ecosystem (TME) and in a simultaneous field-validation study. Experiments were performed on four different European soils in Germany, The Netherlands, United Kingdom and Portugal. Data a...
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