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On the mechanisms of internalization and intracellular delivery mediated by pH-...
Simões, Sérgio; Slepushkin, Vladimir; Düzgünes, Nejat; Pedroso de Lima, Maria C.We investigated the molecular mechanisms by which pH-sensitive liposomes surpass the cytoplasmic and endosomal membranes to deliver their aqueous contents into the cytoplasm. Various liposome formulations were evaluated for their efficacy to mediate intracellular delivery of encapsulated material, including a novel sterically stabilized pH-sensitive formulation ((DOPE:CHEMS:DSPE-PEG2000 (6:4:0.3)) that was prev...
Human serum albumin enhances DNA transfection by lipoplexes and confers resista...
Simões, Sérgio; Slepushkin, Vladimir; Pires, Pedro; Gaspar, Rogério; Pedroso de Lima, Maria C.; Düzgünes, NejatCationic liposome-DNA complexes ([`]lipoplexes') are used as gene delivery vehicles and may overcome some of the limitations of viral vectors for gene therapy applications. The interaction of highly positively charged lipoplexes with biological macromolecules in blood and tissues is one of the drawbacks of this system. We examined whether coating cationic liposomes with human serum albumin (HSA) could generate ...
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