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A novel xylan degrading β-D-xylosidase : purification and biochemical character...
Michelin, Michele; Peixoto-Nogueira, Simone C.; Silva, Tony M.; Jorge, João A.; Terenzi, Héctor F.; Teixeira, J. A.; Polizeli, Maria de Lourdes T. M.Aspergillus ochraceus, a thermotolerant fungus isolated in Brazil from decomposing materials, produced an extracellular b-xylosidase that was purified using DEAE-cellulose ion exchange chromatography, Sephadex G-100 and Biogel P-60 gel filtration. b-xylosidase is a glycoprotein (39 % carbohydrate content) and has a molecular mass of 137 kDa by SDS-PAGE, with optimal temperature and pH at 70 C and 3.0–5.5, respe...
Data: 2012
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Origem: RepositóriUM - Universidade do Minho
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