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Nanostructured 3D constructs based on chitosan and chondroitin sulphate multila...
Silva, Joana M.; Georgi, N.; Costa, Rui; Praveen, S.; Reis, R. L.; Blitterswijk, C. A. van; Karperien, M.; Mano, J. F.Nanostructured three-dimensional constructs combining layer-by-layer technology (LbL) and template leaching were processed and evaluated as possible support structures for cartilage tissue engineering. Multilayered constructs were formed by depositing the polyelectrolytes chitosan (CHT) and chondroitin sulphate (CS) on either bidimensional glass surfaces or 3D packet of paraffin spheres. 2D CHT/CS multi-layered...
Nanostructured hollow tubes based on chitosan and alginate multilayers
Silva, Joana M.; Duarte, Ana Rita C.; Custódio, Catarina; Sher, Praveen; Neto, Ana I.; Pinho, A. C. Marques de; Fonseca, Jaime C.; Reis, R. L.The design and production of structures with nanometer-sized polymer fi lms based on layer-by-layer (LbL) are of particular interest for tissue engineering since they allow the precise control of physical and biochemical cues of implantable devices. In this work, a method is developed for the preparation of nanostructured hollow multilayers tubes combining LbL and template leaching. The aim is to produce hollo...
Nanostructured hollow tubes based on chitosan and alginate multilayers
Silva, Joana M.; Duarte, Ana Rita C.; Custódio, Catarina; Sher, Praveen; Neto, Ana I.; Reis, R. L.; Mano, J. F.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The design and production of structures with nanometer-sized polymer films based on layer-by-layer (LbL) are of particular interest for tissue engineering since they allow the precise control of physical and biochemical cues, as well as the recreation of the natural complexity of ECM. In this work, we develop a met...
Fish scales patterning guiding hASC growth
Silva, Joana M.; Afonso, David Losa de Areia; Silva, Tiago H.; Volpato, F.; Motta, A.; Marques, A. P.; Mano, J. F.; Reis, R. L.; Migliaresi, C.Marine organisms and materials exhibit interesting properties for their use in biomedical applications, following biomimetic approaches [1,2]. For instance, the white seabass (Lates Calcarifer) scales exhibit a very interesting pattern, which inspire their use as a guiding platform for cellular growth, in a tissue regeneration approach. Moreover, fish scales are composed of hydroxyapatite and type I collagen fi...
Marine sponges as natural scaffolds : decellularization by supercritical fluid ...
Duarte, Ana Rita C.; Silva, Joana M.; Silva, Tiago H.; Osinga, R.; Ilan, M.; Marques, A. P.; Mano, J. F.; Reis, R. L.Marine sponges possess remarkable structures, with highly interconnected pores, making them excellent candidates as nature made scaffolds with potential use in tissue engineering strategies. In addition they also exhibit interesting high swelling degree and a skeleton composed mainly of collagen fibres, in some species with embedded siliceous or carbonate spicules. In the present study, several marine sponges (...
A influência da fixação na demonstração do glicogénio
Gasalho, Ana T.; Silva, Joana M.; Mendes, Margarida S.; Ladeira, CarinaA fixação é fundamental para a preservação dos tecidos e é o primeiro passo de uma técnica histológica de rotina cujo objectivo final é obter uma boa visualização das estruturas e, no caso da anatomia patológica, um diagnóstico. Algumas substâncias presentes nos tecidos, como é o caso do glicogénio, necessitam de uma solução fixadora específica capaz de prevenir a dispersão das suas moléculas, sendo a fixação d...
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