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Genotypic and pheno-metabolomic characterization of a Saccharomyces cerevisiae ...

The objective of the present work was to gain a deeper understanding of the phenotypic diversity of natural isolates of Saccharomyces cerevisiae, using high-throughput methods in combination with bioanalytical data. A S. cerevisiae strain collection was constituted comprising 187 strains from different geographical origins and technological uses. Some microsatellite alleles and phenotypes were identified as res...

Characterization of wine fermentations using fiber optic-mediated UV-VIS-SWNIR ...

Spectroscopy is widely used in biological sciences, being applied to liquids, pastes, powders, films, fibers, gases and surfaces. It makes possible to characterize proteins, peptides, lipids, membranes and carbohydrates in pharmaceuticals, foods, plants or animal tissues. It can also provide detailed information about the structure and mechanism of action of molecules. UV-VIS-SWNIR spectroscopy has not been use...

The genotypic and pheno-metabolomic landscape of a Saccharomyces cerevisiae str...

Concepção e desenvolvimento de sistemas biotecnológicos

In a simple and succinct form the authors intend to present the research and development activities that are being undertaken at the University of Minho in the area of biotechnology applied to the study, design, development and construction of equipment goods, which are being carried out together with important Iberian and European industrial companies. The projects are as follows: • Real time monitoring of bee...

Determinantes da dor e do desconforto durante a mamografia

Objectivo: Medir a intensidade de dor e desconforto aquando da mamografia, e identificar os determinantes associados a estes sintomas. Métodos: 150 utentes responderam a um questionário pré e pós exame, que permitiu quantificar a dor e desconforto durante a mamografia, e encontrar os determinantes relacionados com estes sintomas. Resultados: 88% referiu dor na realização do exame, a ansiedade foi um factor prep...

Yeast metabolic state identification by fiber optics spectroscopy

In this manuscript we explore the feasibility of using LWUV-VIS-SWNIR (340 - 1100 nm) spectroscopy to classify Saccharomyces cerevisiae colony structures in YP agar and YPD agar, under different growth conditions, such as: i) no alcohol; ii) 1 % (v/v) Ethanol; iii) 1 % (v/v) 1-Propanol; iv) 1 % (v/v) 1- butanol; v) 1 % (v/v) Isopropanol; vi) 1 % (v/v) (±)-1-Phenylethanol; vii) 1 % (v/v) Isoamyl alcohol; viii) 1...

Endothelin-1 receptors play a minor role in the protection against acute Trypan...

Chagas' disease, caused by the protozoan Trypanosoma cruzi, is a major cause of cardiovascular disability in countries where it is endemic. Damage to the heart microvasculature has been proposed to be an important factor in the pathogenesis of heart dysfunction. Endothelin-1 (ET-1) is a potent vasoconstrictor and exerts its effects via specific ET A and ET B receptors. A few studies have suggested a role for ET...

Feasibility of yeasts and fungi monitoring during cheese maturation using fibre...

Infection by caprine arthritis-encephalitis virus in Rio Grande do Norte State

The prevalence of caprine arthritis-encephalitis (CAE) infection in dairy goat herds from Rio Grande do Norte State, Brazil, was estimated, and the predisposing factors to the infection were identified. An epidemiologic survey was held to collect information on educational level of the owner and origin of the animals, and on sanitation and vaccination practices, diseases, morbidity and mortality rates in the he...

The role of IL-12 in experimental Trypanosoma cruzi infection

Host resistance to Trypanosoma cruzi infection is dependent on both natural and acquired immune responses. During the early acute phase of infection in mice, natural killer (NK) cell-derived IFN-<FONT FACE="Symbol">g</font> is involved in controlling intracellular parasite replication, mainly through the induction of nitric oxide biosynthesis by activated macrophages. We have shown that IL-12, a powerful induce...