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Key stress factors and parameters for batch production optimisation of silk-ela...

Collins, Tony; Silva, J. Azevedo; Costa, A.; Machado, Raul; Casal, Margarida


Creating new functional biomaterials : construction and production of Bone Morp...

Silva, J. Azevedo; Machado, Raul; Reis, R. L.; Rodríguez-Cabello, José Carlos; Casal, Margarida

Bone morphogenetic protein 2 (BMP-2) is a potent osteoinductive cytokine from the TGF-β superfamily that triggers the development of stem cells into osteoblasts. Its therapeutic interest has led to the development of various production systems for recombinant variables of BMP-2. Production has been achieved in expression systems ranging from animal cells to bacteria, but is always associated with three major dr...


Use of elastin like polymer (VPAVG)220 fusion TAGS for recombinant protein, pro...

Silva, J. Azevedo; Costa, A.; Machado, Raul; Rodríguez-Cabello, José Carlos; Casal, Margarida

One of the major challenges to the economical preparation of biomedical and biotechnological recombinant proteins today resides in the availability of an efficient production and purification protocol. Some proteins may not lend themselves to effective purification or may require expensive and cumbersome multistep chromatographic protocols. On the other hand, use of synthetically produced protein based polymer ...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia