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A profissionalização em Educação Pré-Escolar e no 1.º Ciclo do Ensino Básico
Silva, Carla ManuelaO presente relatório foi realizado no âmbito do Mestrado em Educação Pré- Escolar e em Ensino do 1.º Ciclo do Ensino Básico. Este foi realizado numa perspetiva de progresso pessoal e profissional, fomentando uma postura crítica e reflexiva, apoiando-se numa constante reflexão. O estágio em Educação Pré-Escolar foi realizado numa instituição Particular de Solidariedade Social e o do 1º Ciclo do Ensino Básico num...
Odorant binding proteins : a biotechnological tool for odour control
Silva, Carla Manuela; Matamá, Maria Teresa; Azoia, Nuno G.; Mansilha, Catarina; Casal, Margarida; Paulo, Artur CavacoThe application of an odorant binding protein for odour control and fragrance delayed release from a textile surface was first explored in this work. Pig OBP-1 gene was cloned and expressed in Escherichia coli , and the purified protein was biochemically characterized. The IC50 values(concentrations of competitor that caused a decay of fluorescence to half-maximal intensity) were determined for four distinct fr...
Functionalization of gauzes with liposomes entrapping an anti-inflammatory drug...
Ferreira, Helena; Matamá, Maria Teresa; Silva, Raquel; Silva, Carla Manuela; Gomes, A. C.; Paulo, Artur CavacoFrom ancientness, suitable materials have been developed to cover the wounds in order to prevent infections and promote proper wound healing. In this study, the successful development of functionalized nonwoven gauzes with liposomes entrapping anti-inflammatory piroxicam is reported. Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) that can suppress a persistent inflammatory response, leading to impr...
Sonochemical and hydrodynamic cavitation reactors for laccase/hydrogen peroxide...
Gonçalves, Idalina; Martins, Madalena A.; Loureiro, A.; Gomes, A. C.; Paulo, Artur Cavaco; Silva, Carla ManuelaArticle in press ; The main goal of this work is to develop a novel and environmental-friendly technology for cotton bleaching with reduced processing costs. This work exploits a combined laccase–hydrogen peroxide process assisted by ultrasound. For this purpose, specific reactors were studied, namely ultrasonic power generator type K8 (850 kHz) and ultrasonic bath equipment Ultrasonic cleaner USC600TH (45 kHz...
Bullying
Silva, Carla Manuela Barbosa daTrabalho apresentado à Universidade Fernando Pessoa como parte dos requisitos para a obtenção do grau de Licenciada em Criminologia ; O presente trabalho aborda a problemática do fenómeno do bullying na Escola Profissional de Comércio Externo, situada no centro da cidade do Porto. O estudo conta com uma amostra de 95 jovens, com idades compreendidas entre os 15 e os 18 anos de idade. O instrumento utilizado pa...
Bamboo fibre processing : insights into hemicellulase and cellulase substrate a...
Fu, Jiajia; Nyanhongo, Gibson S.; Silva, Carla Manuela; Silva, Carla Manuela; Silva, Carla Manuela; Cardinale, Massimiliano; Prasetyo, E. N.A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo materia...
Decolourization of paprika dye effluent with hydrogen peroxide produced by gluc...
Gonçalves, Idalina; Silva, Carla Manuela; Paulo, Artur CavacoHydrogen peroxide was produced from bran by a two-step process using cellulase/xylanase and glucose oxidase, sequentially. The decolourization efficiency of the produced reagent was tested using paprika oil dye (effluent from industrial source) and high levels of colour removal (96%) were achieved after saponification pre-treatment and hydrogen peroxide application. The method is economically and environmentall...
Molecular recognition of esterase plays a major role on the removal of fatty so...
Silva, Carla Manuela; Azoia, Nuno G.; Martins, Madalena A.; Matamá, Maria Teresa; Wu, J.; Paulo, Artur CavacoIn this work it is describe for the first time, the use of an esterase with null activity (Tfu_0883 bacterial cutinase from Thermobifida fusca) on the removal of fat from the surface of a cotton substrate. Similar levels of fat removal were found for both null and wild-type proteins, despite that only wild type protein yielded fatty acids. Our results show that molecular recognition of esterase plays a major ro...
Polyoxometalate/laccase-mediated oxidative polymerization of catechol for texti...
Kim, Suyeon; Silva, Carla Manuela; Evtuguin, D. V.; Gamelas, José A. F.; Paulo, Artur CavacoThe synergistic effect between polyoxometalates (POMs), namely K5[SiW11VVO40]·11H2O and H5[PMo10 VV2O40]·13H2O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the formation of a relatively high molecular weight polycatechol as confirmed by size exclusion chromatography and electrospray ion...
In situ laccase-assisted overdyeing of denim using flavonoids
Guimarães, Clara; Kim, Suyeon; Silva, Carla Manuela; Paulo, Artur CavacoA laccase-mediated system for denim overdyeing using phenolic compounds was developed. Laccase from ascomycete Myceliophthora thermophila was able to oxidize phenolic compounds such as catechol and catechin and mediate their attachment to denim surfaces. Laccase-generated polymers gave rise to new coloration states from dark brown to green–yellow and replaced dyes in the overdyeing process. Process parameters, ...
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