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Preparation and characterization of polysaccharides/PVA blend nanofibrous membr...
Santos, Carla; Silva, Carla J.; Büttel, Zsófia; Guimarães, Rodrigo; Pereira, Sara B.; Tamagnini, Paula; Zille, AndreaA series of polyvinyl alcohol (PVA), PVA/chitosan (CS) and PVA/cyanobacterial extracellular polymeric substances (EPS) blended nanofibrous membranes were produced by electrospinning using a microfiltration poly(vinylidene fluoride) (PVDF) basal membrane, for potential applications in water filtration. Nanofibres were obtained from solutions of 20% (w/w) PVA with 1% (w/w) CS or EPS, using a weight ratio of 60/40...
New biotextiles for tissue engineering : development, characterization and in v...
Almeida, L. R.; Martins, A. R.; Fernandes, E. M.; Oliveira, Mariana B.; Correlo, V. M.; Pashkuleva, I.; Marques, A. P.; Ribeiro, Ana S.This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparativ...
Alkali and acid polysaccharides blend nanofibrous membranes prepared by electro...
Santos, Carla; Silva, Carla J.; Büttel, Zsófia; Guimarães, Rodrigo; Tamagnini, Paula; Zille, AndreaPoster apresentado no "Smart and functional coatings conference", Torino, Italy, 2013 ; Electrospinning allows the production of polymer fibres with diameters in the sub-micron size range, through the application of an external electric field, keeping intact the bulk properties of the polymers. Electrospun membranes possess some unique structural features, such as a high surface to volume ratio and very good m...
Preparation and characterization of polysaccharides/PVA blend nanofibrous membr...
Santos, Carla; Silva, Jose; Silva, Carla J.; Zille, AndreaApresentação efetuada no Smart & Funcional Coatings–European Conference Turin, 26th September 2013 ; A polyvinyl alcohol (PVA) / cyanobacterial EPS blend nanofibrous membranes were fabricated by electrospinning using polyvinylidene fluoride (PVDF) as a basal membrane, in order to obtain thin-layer composite nanofiltration membranes. The production of the nanofibers using EPS and PVA as plasticizer in different...
Fabrication and characterization of PVA, PVA/chitosan, and PVA/cyanobacterial e...
Santos, Carla; Silva, Carla J.; Guimarães, Rodrigo; Büttel, Zsófia; Tamagnini, Paula; Zille, AndreaApresentação efetuada no "245th ACS National Meeting and Exposition", em New Orleans, Louisiana, 2013 ; A series of poly(vinyl alcohol) (PVA), PVA/chitosan (CS) and PVA/cyanobacterial exopolysaccharide (EPS) blend nanofibrous membranes were fabricated by electrospinning using a microfiltration poly(vinylidene fluoride) (PVDF) as a basal membrane, in order to obtain thin-layer composite (TFC) nanofiltration mem...
Thin-layer nanofiltration membranes using engineered biopolymers for seawater d...
Büttel, Zsófia; Guimarães, Rodrigo; Santos, Carla; Silva, Carla J.; Pereira, Sara B.; Mota, Rita; Tamagnini, Paula; Zille, AndreaNowadays water demand already exceeds supply and water scarcity is a global problem. So it is necessary to develop novel technologies to be able to use poorer quality source waters for drinking water production. Once considered as an expensive, ultimate solution for water supply, desalination is becoming affordable. The two most commonly used seawater desalination methods are Multi-stage Flash Distillation (MSF...
Silk-based 3D biotextiles support human adipose derived stem cells towards oste...
Ribeiro, V. P.; Ribeiro, Ana S.; Silva, Carla J.; Durães, Nelson F.; Bonifácio, Graça; Marques, A. P.; Sousa, R. A.; Oliveira, A. L.; Reis, R. L.Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The present work aims at evaluating the potential of recently developed 3D silk-based biotextile structures to support hASCs adhesion, proliferation and osteogenic differentiation. The 3D spacer structures were processed by using a knitting technology. Two knitted silk layers were assembled and spaced by a monofila...
Production and characterization of extracellular carbohydrate polymer from Cyan...
Mota, Rita; Guimarães, Rodrigo; Büttel, Zsófia; Rossi, Federico; Colica, Giovanni; Silva, Carla J.; Santos, Carla; Gales, Luís; Zille, AndreaCyanobacterial extracellular polymeric substances (EPS) are heteropolysaccharides that possess characteristics suitable for industrial applications, notably a high number of different monomers, strong anionic nature and high hydrophobicity. However, systematic studies that unveil the conditions influencing EPS synthesis and/or its characteristics are mandatory. In this work, Cyanothece sp. CCY 0110 was used as ...
Stability and decolourization ability of trametes villosa laccase in liquid ult...
Basto, Carlos; Silva, Carla J. S. M.; Gübitz, Georg M.; Paulo, Artur CavacoWe report in this study that the sonication of laccase from Trametes villosa and bovine serum albumin promotes the formation of protein aggregates with high molecular weight. The formation of aggregates leads to the deactivation of the enzyme, fact that was confirmed by the analysis of the enzyme stability (half-life time) upon ultrasound treatment. This inactivation was mainly caused by the radicals formed by ...
Cotton fabric : a natural matrix suitable for controlled release systems
Todorova, Snezhana Bogomilova; Silva, Carla J. S. M.; Simeonov, Nikolay Petkov; Paulo, Artur CavacoThe possibility to use cotton as a matrix for controlled release systems was studied by covalently attaching a model compound, specifically the reactive dye Remazol Brilliant Blue R to its surface. Afterwards the fabric was coated with a commercial cellulase. The release of the dye, obtained by the hydrolysis of cotton fibres in sweat buffer, was monitored. The reducing sugars concentration increased for both f...
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