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Protein micro- and nano-capsules for biomedical applications
Shimanovich, Ulyana; Bernardes, Gonçalo J. L.; Knowles, T. P. J.; Paulo, Artur CavacoMicro- and nano-scale systems have emerged as important tools for developing clinically useful drug delivery systems. In this tutorial review, we discuss the exploitation of biomacromolecules for this purpose, focusing on proteins, polypeptides, nucleic acids and polysaccharides and mixtures thereof as potential building blocks for novel drug delivery systems. We focus on the mechanisms of formation of micro- a...
In vitro and computational studies of transdermal perfusion of nanoformulations...
Martins, M.; Azoia, Nuno G.; Ribeiro, Artur J.; Shimanovich, Ulyana; Silva, Carla; Paulo, Artur CavacoTransdermal perfusion of a large protein is reported for the first time, using a nanoemulsion of bovine serum albumin (66 kDa) of 160 nm prepared by a solid-in-oil (S/O) process. Molecular dynamics simulations confirmed skin permeation by these formulations, with integration of the protein into the lipid bilayers. These results demonstrate the real possibility of delivering large proteins transdermally for a ra...
The immobilization of polyethylene imine nano and microspheres on glass using h...
Shimanovich, Ulyana; Paulo, Artur Cavaco; Gedanken, AharonThe present article describes the creation and immobilization of Polyethylene imine (PEI) capsules on a glass surface. The synthesis and deposition were accomplished by short-time (3 min) one-step reaction. The preparation and immobilization of PEI spheres, was carried out using an environmental friendly method, the ultrasonic emulsification. The ultrasonic technique enables to control size and fulfillment of t...
Keratins and lipids in ethnic hair
Cruz, Célia F.; Fernandes, Margarida M.; Gomes, Andreia; Coderch, L.; Martí, M.; Méndez, Sandra; Gales, Luís; Azoia, Nuno G.; Shimanovich, UlyanaHuman hair has an important and undeniable relevance in society due to its important role in visual appearance and social communication. Hair is mainly composed of structural proteins, mainly keratin and keratin associated proteins, and lipids. Herein, we report a comprehensive study of the content and distribution of the lipids among ethnic hair, African, Asian and Caucasian hair. More interestingly, we also r...
Proteinaceous microspheres for targeted RNA delivery prepared by an ultrasonic ...
Shimanovich, Ulyana; Eliaz, Dror; Zigdon, Sally; Volkov, Vadim; Aizer, Adva; Paulo, Artur Cavaco; Michaeli, S.; Shav-Tal, Yaron; Gedanken, AharonIn the present work we used sonochemically prepared proteinaceous BSA spheres as a novel RNA-delivery system. The preparation of RNA-loaded BSA spheres was accomplished using an environmental friendly method termed the “ultrasonic emulsification method”. It was demonstrated that ultrasonic waves do not cause the RNA chains to degrade and the RNA molecules remain untouched. The BSA–RNA complex was successfully i...
Sonochemical coating of cotton and polyester fabrics with “Antibacterial” BSA a...
Nitzan, Yeshayahu; Paulo, Artur Cavaco; Shimanovich, Ulyana; Gedanken, AharonA novel antibacterial coating for cotton and polyester fabrics has been developed by using drug-loaded proteinaceous microspheres made of bovine serum albumin and casein proteins. The microbubbles were created and anchored onto the fabrics (see figure) in a one-step reaction that lasts 3 min. The sonochemically produced “antibacterial fabrics” have been characterized. The efficiency of the sonochemical process ...
Releasing dye encapsulated in proteinaceous microspheres on conductive fabrics ...
Shimanovich, Ulyana; Perelshtein, Ilana; Paulo, Artur Cavaco; Gedanken, AharonThe current paper reports on the relase properties of conductive fabrics coated with proteinaceous microspheres containing a dye. The release of the dye was achieved by passing an electric current through the fabric. The conductivity of the polyester fibers resulted from nanosilver (Ag NPs) coated on the surface of these fibers. Both types of coatings (nanosilver coating and the coating of the proteinaceous mic...
Insights on the mechanism of formation of protein microspheres in a biphasic sy...
Silva, Raquel; Ferreira, Helena; Azoia, Nuno G.; Shimanovich, Ulyana; Freddi, G.; Gedanken, Aharon; Paulo, Artur CavacoMicrospheres of bovine serum albumin (BSA) and silk fibroin are produced by applying ultrasound in a biphasic system consisting of an aqueous protein solution and an organic solvent. The protein microspheres are dispersed in an aqueous media where the protein remains at the interface covering the organic solvent. This only occurs when high shear forces are applied that induce changes to force the protein to the...
Encapsulation of RNA molecules in BSA microspheres and internalization into Try...
Shimanovich, Ulyana; Tkacz, Itai Dov; Eliaz, Dror; Paulo, Artur Cavaco; Michaeli, S.; Gedanken, AharonRNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water–oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA,...
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