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Honey bees (Hymenoptera: Apidae) of african origin exist in non-africanized are...
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Schiff, Nathan M.; Kandemir, Irfan; Patton, John C.Descendents of Apis mellifera scutellata Lepeletier (Hymenoptera: Apidae) (the Africanized honey bee) arrived in the United States in 1990. Whether this was the Þrst introduction is uncertain. A survey of feral honey bees from non-Africanized areas of the southern United States revealed three colonies (from Georgia, Texas, and New Mexico) with a diagnostic African mitochondrial DNA cytochrome b/BglII fragment p...
Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A....
Kandemir,Irfan; Pinto,Maria A.; Meixner,Marina D.; Sheppard,Walter S.Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I ...
Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-...
Kandemir, Irfan; Pinto, M. Alice; Meixner, Marina D.; Sheppard, Walter S.Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I ...
Evidence of African honey bees mitotypes in the southern United States prior to...
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.The standard protocol for assessing honey bees with a mitotype derived from an African origin is the amplification of a segment of cytochrome b and subsequent digestion of the amplified fragment with the restriction enzyme Bgl II. A previous survey of 451pre-Africanization honey bees from the southern U. S. revealed three bees with restriction pattern consistent with an African mitotype. To confirm these bees t...
Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA...
Pinto, M. Alice; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.; Sheppard, Walter S.Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all know...
Analysis of Africanized honey bee mitochondrial DNA reveals further diversity o...
Sheppard,Walter S.; Rinderer,Thomas E.; Garnery,Lionel; Shimanuki,HachiroWithin the past 40 years, Africanized honey bees spread from Brazil and now occupy most areas habitable by the species Apis mellifera, from Argentina to the southwestern United States. The primary genetic source for Africanized honey bees is believed to be the sub-Saharan honey bee subspecies A. m. scutellata. Mitochondrial markers common in A. m. scutellata have been used to classify Africanized honey bees in ...
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