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Development and industrialisation of enzymatic shrink-resist process based on m...

Shen, J.; Rushforth, Mike; Paulo, Artur Cavaco; Guebitz, G. M.; Lenting, Herman

There is currently considerable interest in the use of enzymes to achieve a variety of finishing effects on wool, but it is apparent that the extent of fibre degradation by enzymes is of major concern during their commercial application. Proteolytic enzymes are known to penetrate and degrade the internal wool structure during processing, causing fibre damage, rather than limiting the degradation to the cuticle ...


Immobilization of proteases with a water soluble–insoluble reversible polymer f...

Silva, Carla J. S. M.; Zhang, Qinghua; Shen, J.; Paulo, Artur Cavaco

A commercial protease, Esperase, was covalently linked to Eudragit S-100, a reversible soluble–insoluble polymer by carbodiimide coupling. When compared to the native enzyme, the immobilized form presented a lower specific activity towards high molecular weight substrates but a higher thermal stability at all temperatures tested. The optimum pH of the immobilized protease was shifted towards the alkaline side b...


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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia