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Multilocus sequence identification of penicillium species in cork bark during p...

Despite several studies reporting Penicillium as one of the most frequent fungal genera in cork planks, the isolates were rarely identified to species level.We conducted a detailed study to identify Penicillium species from the field to the factory environment prior to and after boiling the cork planks. A total of 84 samples were analyzed. Of the 486 Penicillium isolates phenotypically identified, 32 representa...

Penicillium astrolabium and penicillium neocrassum, two new species isolated fr...

We describe two new terverticillate Penicillium species isolated from grapes on the basis of phenotypic and phylogenetic differences from known species. The strains were isolated in the course of a study to establish the mycobiota of grapes in Portugal. Penicillium astrolabium is phenotypically similar to P. olsonii but differs from it by two cultural characters, growth rates and the colony reverse color. P. ne...

Microbial strain characterisation by MALDI-TOF MS - possibilities and limits

Ochratoxin A occurrence and formation in Portuguese wine grapes at various stag...

A total of 93 Portuguese grape samples destined for wine production were examined for the presence of ochratoxin A (OTA) and the OTA producing fungi Aspergillus carbonarius and A. niger aggregate. Samples came from 11 vineyards from four winemaking regions in the North and South of the Portuguese mainland, during the harvest seasons of 2001, 2002 and 2003. Grapes were examined at 3 maturation stages, from setti...

Influence of the region of origin on the mycobiota of grapes with emphasis on a...

A three-year study was undertaken to investigate the fungal species present on the surface of grape berries from Portuguese vineyards in four winemaking regions. Emphasis was given to Aspergillus and Penicillium species due to their relevance for mycotoxin production. From the 3517 fungal strains detected 27 genera were identified. The region of origin markedly influenced the spoilage fungal population to which...

Aspergillus ibericus : a new species of section nigri characterised by MALDI-TO...

Strains from the new described species Aspergillus ibericus were characterised using MALDI-TOF MS and the results were compared with other related species of section Nigri.

Application of classification-tree models to characterize the mycobiota of grap...

Classification-tree(CT) models are a simple and robust exploratory data analysis technique that can be used in classification, regressions and summaries of data. They distill complex ecological relationships into simplified rules and identify the species necessary for sample classification on the basis of detailed ecological inventories. The usefulness of this technique to characterize and represent differences...

Fungi and ochratoxin A detected in healthy grapes for wine production

Aims: The mycoflora of healthy grapes (i.e. without visible symptoms of rot) for wine production in Portuguese winemaking regions was assessed and its potential for ochratoxin A (OTA) production evaluated. The OTA content of grapes was also determined. Methods and Results: A total of 386 fungal strains were isolated by plating methods. The most frequent genera found in grapes were non-ochratoxigenic species: Cl...

Micoflora e micotoxinas em uvas

Aspergillus ibericus : a new species of the section Nigri isolated from grapes