The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the ...
Aqueous two-phase systems (ATPSs) composed by UCON (ethylene oxide/propylene oxide copolymer) and potassium phosphate salts were for the first time evaluated in the recovery of Peniophora cinerea laccase from complex fermented medium. The ATPSs were obtained by combining the random copolymer UCON with KH2PO4, potassium phosphate buffer pH 7 or K2HPO4. According to the results, protein partition occurred predomi...
The aim of this work was to assess the possibility of using beer spent grain (a byproduct of beer’s brewing industry) as a carrier for laccase immobilization. Both adsorption (on spent grain – SG and on digested spent grain – DSG) and covalent binding (using glycidol and glycidol followed by ethylenediamine on DSG) were used. The effect of different immobilization conditions on the immobilization yields and rec...
The interference of some specific aqueous two-phase system (ATPS) phase-forming components in bovine serum albumin (BSA) determination by the Bradford method was investigated. For this purpose, calibration curves were obtained for BSA in the presence of different concentrations of salts and polymers. A total of 19 salts [Na(2)SO(4), (NH(4))(2)SO(4), MgSO(4), LiSO(4), Na(2)HPO(4), sodium phosphate buffer (pH 7.0...
Solubility has been recognized as one of the most important properties for designing separation and purification processes of complex molecules, such as active pharmaceutical ingredients. Experimental solubility data are usually needed for performing such design operations. However, frequently data are unavailable due to reduced amounts of sample, time limitations, or inherent complexities with experimental mea...
Since their isolation in the 19th century the physical and chemical properties of amino acids became a very important studied subject, no only because of their value as basic elements in all forms of life but also for their industrial importance, particularly for food. Chemical, medical, pharmaceutical and cosmetics industries.
Phenolic compounds represent a class of important chemicals with both biological and industrial importance. Their production, either by chemical synthesis or extraction from different biological media requires the adequate knowledge of phase equilibria. Particularly, the solubility in aqueous systems organic and supercritical solvents are fundamental for a better design of separation and purification processes.
Phenolic compounds are relevant chemicals in industrial and biological processes. Their production, either by synthesis or extraction from biological media, requires the knowledge of phase equilibrium data that is lacking considerably in the open literature. For this reason, we have been performing at our laboratory a series of experimental measurements of solubility in water (1,2] and organic solvents, focused...
Phenolic compounds are well known for their biological activity and, due to their reactivity, are important starting materials for the synthesis of complex molecules. These compounds can be obtained either by chemical synthesis or by extraction from different biological media. Thus, solubility in aqueous systems, organic and supercritical solvents are fundamental for a better design of reaction, separation and ...
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