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A possible approach for gel-based proteomic studies in recalcitrant woody plants
Sebastiana, Mónica; Figueiredo, Andreia; Monteiro, Filipa; Martins, Joana; Franco, Catarina; Coelho, Ana Varela; Vaz, Fátima; Simões, TâniaWoody plants are particularly difficult to investigate due to high phenolic, resin, and tannin contents and laborious sample preparation. In particular, protein isolation from woody plants for two-dimensional gel electrophoresis (2-DE) is challenging as secondary metabolites negatively interfere with protein extraction and separation. In this study, three protein extraction protocols, using TCA, phenol and etha...
Global characterization of the Quercus suber ectomycorrhizal transcriptome usin...
Sebastiana, Mónica; Neto, T. Lino; Tavares, R. M.; Pais, Maria SaloméComunicação em painel ; In temperate forests, trees live in symbiosis with fungi and depend on them for their survival. Species from the Fagaceae family, including oaks, establish a symbiotic relationship in their roots with basidiomycetous fungi, called ectomycorrhizas (ECM). ECM symbiosis is essential for the life and health of trees in temperate and boreal forests where it plays a major role in nutrient cyc...
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