Encontrados 3 documentos, a visualizar página 1 de 1
Hydroxylation of polypropylene using the monooxygenase mutant 139-3 from Bacill...
Hasmann, Andrea; Eberl, Anita; Schneider, Konstantin; Schoening, K.-U.; Kaufmann, Franz; Glieder, Anton; Kovac, Janez; Paulo, Artur CavacoEnzymatic hydroxylation of polypropylene (PP) was investigated in order to increase hydrophilicity. A mutant (139-3) of the P450monooxygenase from Bacillus megaterium expressed in E. coli DH5α was purified using anion exchange chromatography. Hydroxylation of PP fabrics led to a dramatic increase of hydrophilicity as indicated by a water drop dissipation time of below 1 s compared to the hydrophobic reference m...
Protein disulphide isomerase-assisted functionalization of keratin-based matrices
Fernandes, Margarida M.; Gomes, A. C.; Vasconcelos, Andreia; Munteanu, Florentina-Daniela; Tzanov, Tzanko; Gonçalves, M. Sameiro T.; End, N.In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (isomerase activity), leading proteins towards their native configuration. In this study, PDI was used to attach cysteine-containing compounds (CCCs) onto hair, to enhance compound migration within hair fib...
Enzymatic synthesis of Tinuvin
Schroeder, M.; Pereira, Luciana; Rodríguez Couto, S.; Erlacher, Angelika; Schoening, K.-U.; Paulo, Artur Cavaco; Guebitz, G. M.Coupling of 3-(3-tert-butyl-4-hydroxyphenyl) propionic acid methylester to 1H-benzotriazole using a laccase from Trametes hirsuta was studied. The potentially resulting coupling product Tinuvin 1130 is an important UV-absorber used in polymer based materials. Oxidation of the phenol by the laccase led to homomolecular coupling reactions while the laccase did not attack 1H-benzotriazole. Due to the homomolecular...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||