Encontrados 7 documentos, a visualizar página 1 de 1
Energy Metabolism in Human Pluripotent Stem Cells and Their Differentiated Coun...
Varum, Sandra; Rodrigues, Ana S.; Moura, Michelle B.; Momcilovic, Olga; Easley, Charles A.; Ramalho-Santos, João; Van Houten, Bennett; Schatten, GeraldBackground: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless their use may prove challenging as incomplete differentiation of these cells might lead to tumoregenicity. Interestingly, many cancer types have been reported ...
LIS1 association with dynactin is required for nuclear motility and genomic uni...
Payne, Christopher; John, Justin C. St.; Ramalho-Santos, João; Schatten, GeraldMutations in the human LIS1 gene cause the devastating brain disorder lissencephaly. LIS1 also regulates microtubule dynamics; it interacts with the molecular motor cytoplasmic dynein and its cofactor dynactin, and is necessary for neuronal migration. Recently, LIS1 has been suggested to mediate pronuclear migration during fertilization. Here we use rhesus monkey and bovine oocytes, as well as pronucleate-stage...
VAMP/synaptobrevin as an acrosomal marker for human sperm
Ramalho-Santos, João; Terada, Yukihiro; Schatten, GeraldObjective: To determine the possible use of the mammalian acrosomal marker vehicle-associated membrane protein (VAMP)/synaptobrevin to detect acrosome abnormalities in human sperm. ; http://www.sciencedirect.com/science/article/B6T6K-44T0V32-V/1/6442a6b568369477e346d6c367fe5891
Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the...
Moreno, Ricardo D.; Schatten, Gerald; Ramalho-Santos, JoãoWe have studied Golgi apparatus dynamics during mouse oocyte in vitro maturation, employing both live imaging with the fluorescent lipid BODIPY-ceramide and immunocytochemistry using several specific markers (beta-COP, giantin, and TGN38). In germinal vesicle oocytes the Golgi consisted of a series of structures, possibly cisternal stacks, dispersed in the ooplasm, but slightly more concentrated in the interior...
Membrane Trafficking Machinery Components Associated with the Mammalian Acrosom...
Ramalho-Santos, João; Moreno, Ricardo D.; Wessel, Gary M.; Chan, Edward K. L.; Schatten, GeraldActive trafficking from the Golgi apparatus is involved in acrosome formation, both by delivering acrosomal contents to the nascent secretory vesicle and by controlling organelle growth and shaping. During murine spermiogenesis, Golgi antigens (giantin, [beta]-COP, golgin 97, mannosidase II) are detected in the acrosome until the late cap-phase spermatids, but are not found in testicular spermatozoa (maturation...
Acrosome components after intracytoplasmic sperm injection: the decondensation ...
Ramalho-Santos, João; Simerly, Calvin R.; Hewitson, Laura; Schatten, Geraldhttp://www.sciencedirect.com/science/article/B6T6K-43CBFGC-14/1/c122d3f8e7188ef9ec4a133a8068995b
SNAREs in Mammalian Sperm: Possible Implications for Fertilization
Ramalho-Santos, João; Moreno, Ricardo D.; Sutovsky, Peter; Chan, Anthony Wing-Sang; Hewitson, Laura; Wessel, Gary M.; Simerly, Calvin R.Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotag...
| Financiadores do RCAAP | |||||||
|
|
|
|
|
|
||