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Stability studies of a recombinant cutinase immobilized to dextran and derivati...
Gonçalves, A. M.; Schacht, E.; Matthijs, G.; Aires Barros, M. R.; Cabral, J. M. S.; Gil, M. H.Recombinant cutinase from Fusarium solani pisi was covalently attached to dextran and two derivatized silica supports, Biosil-NH2 and Biosil-Dextran-NH2. Kinetic parameters were determined for all three systems as well as for soluble cutinase. Long-term stability in aqueous media was studied; dextran may have a stabilizing role not only due to the covalent links involved but also in the same way as other polyhy...
Physical and chemical characterisation of some silicas and silica derivatives
Ramos, M. A.; Gil, M. H.; Schacht, E.; Matthys, G.; Mondelaers, W.; Figueiredo, M. M.An extensive study of four types of porous silica supports has been performed, with particular emphasis on their physical and morphological characteristics. These silicas were modified by reacting the surface silanol groups present with either 3-(trimethoxysilyl)propylmethacrylate or 3-glycidoxypropyltrimethoxysilane or 3-aminopropyltriethoxysilane, to produce derivatives with suitable functional groups for fur...
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