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In search of synergistic effects in antioxidant capacity of combined edible mus...
Queirós, Bruno; Barreira, João C.M.; Sarmento, Ana C.; Ferreira, Isabel C.F.R.The antioxidant activity of different edible mushrooms was evaluated considering the different contribution of individual and combined extracts. The radical scavenging capacity was evaluated through hydrogen atom transfer and single electron transfer reaction-based assays: DPPH radical scavenging activity and reducing power, respectively. The inhibition of lipid peroxidation was studied in lipossomes solutions ...
Thermostability of cardosin A from Cynara cardunculus L.
Pina, David G.; Oliveira, Claudia S.; Sarmento, Ana C.; Barros, Marlene; Pires, Euclides; Zhadan, Galina G.; Villar, Enrique; Gavilanes, FranciscoThe structural stability of cardosin A, a plant aspartic proteinase (AP) from Cynara cardunculus L., has been investigated by high-sensitivity differential scanning calorimetry, intrinsic fluorescence and circular dichroism spectroscopy, and enzymatic activity assays. Even though the thermal denaturation of cardosin A is partially irreversible, valid thermodynamic data can be obtained within a wide pH region. A...
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