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Effect of the ionic liquid [bmim]Cl and high pressure on the activity of cellulase
Salvador, Ângelo C.; Santos, Mickael Da C.; Saraiva, Jorge A.The effect of the ionic liquid 1-butyl-3-methylimidazolium chloride ([bmim]Cl) and of high pressure on the activity of cellulase from Aspergillus niger were studied separately and in combination. The enzyme activity decreased with increasing concentrations of [bmim]Cl, reaching 50% the value in aqueous buffer with 20% [bmim]Cl. However, when the enzyme is held in 10% [bmim]Cl and is then assayed in 1% [bmim]Cl,...
Trace elements and activity of antioxidative enzymes in Cistus ladanifer L. gro...
Abreu, Maria Manuela; Santos, Erika S.; Nabais, Cristina; Saraiva, Jorge A.Abstract The Mediterranean shrub Cistus ladanifer grows naturally in Sa˜o Domingos (Portugal), an abandoned copper mine. High levels of trace elements in plants can generate oxidative stress increasing the activity of antioxidant enzymes. The aim of this work was to evaluate and compare As, Cu, Pb and Zn concentrations and the activity of the soluble and cell wall ionically bounded forms of the enzymes catalase...
Characterization of Plum Procyanidins by Thiolytic Depolymerization
Nunes, Cláudia; Guyot, Sylvain; Marnet, Nathalie; Barros, António S.; Saraiva, Jorge A.; Renard, Catherine M. G. C.; Coimbra, Manuel A.The phenolic compounds of ?Green Gage? (GG) plums (Prunus domestica L.), ?Rainha Cla?udia Verde?, from a ?protected designation of origin? (PDO), in Portugal, were quantified in both flesh and skin tissues of plums collected in two different orchards (GG-V and GG-C). Analyzes of phenolic compounds were also performed on another GG European plum obtained in France (GG-F) and two other French plums, ?Mirabelle? (...
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