Encontrados 25 documentos, a visualizar página 1 de 3
Human skin cell fractions fail to self-organize within a gellan gum/Hyaluronic ...
Cerqueira, M. T.; Silva, L. P. da; Santos, T. C.; Pirraco, Rogério; Correlo, V. M.; Marques, A. P.; Reis, R. L.Split-thickness autografts still are the current gold standard to treat skin, upon severe injuries. Nonetheless, autografts are dependent on donor site availability and often associated to poor quality neoskin. The generation of dermal–epidermal substitutes by tissue engineering is seen as a promising strategy to overcome this problematic. However, solutions that can be safely and conveniently transplanted in o...
Human adipose stem cells cell sheet constructs impact epidermal morphogenesis i...
Cerqueira, M. T.; Pirraco, R. P.; Santos, T. C.; Rodrigues, D. B.; Frias, A. M.; Martins, A.; Reis, R. L.; Marques, A. P.Among the wide range of strategies to target skin repair/regeneration, tissue engineering (TE) with stem cells at the forefront, remains as the most promising route. Cell sheet (CS) engineering is herein proposed, taking advantage of particular cell-cell and cell-extracellular matrix (ECM) interactions and subsequent cellular milieu, to create 3D TE constructs to promote full-thickness skin wound regeneration. ...
Adipose stem cell-derived osteoblasts sustain the functionality of endothelial ...
Pirraco, Rogério; Ferreira, B. M.; Santos, T. C.; Frias, A. M.; Marques, A. P.; Reis, R. L.Vascularization is the most pressing issue in tissue engineering (TE) since ensuring that engineered constructs are adequately perfused after in vivo transplantation is essential for the construct’s survival. The combination of endothelial cells with current TE strategies seems the most promising approach but doubts persist as to which type of endothelial cells to use. Umbilical cord blood (UCB) cells have been...
In vivo performance of chitosan/soy-based membranes as wound dressing devices f...
Santos, T. C.; Hoering, B.; Reise, Kathrin; Silva, S. S.; Oliveira, Joaquim M.; Mano, J. F.; Castro, António G.; Reis, R. L.; Griensven, Martijn vanWound management represents a major clinical challenge on what concerns healing enhancement and pain control. The selection of an appropriate dressing plays an important role in both recovery and esthetic ap- pearance of the regenerated tissue. Despite the wide range of available dressings, the progress in the wound care market relies on the increasing interest in using natural-based biomedical products. Herein...
VEGF and FGF-2 incorporation in starch-based bone tissue engineering constructs...
Santos, T. C.; Morton, Tatjana J.; Moritz, Martina; Pfeifer, Sabine; Reise, Kathrin; Marques, A. P.; Castro, António G.; Reis, R. L.The ideal bone tissue-engineered (TE) construct remains to be found, although daily discoveries significantly contribute to improvements in the field and certainly have valuable long-term outcomes. In this work, different TE elements, aiming at bone TE applications, were assembled and its effect on the expression of several vas- cularization/angiogenesis mediators analyzed. Starch/polycaprolactone (SPCL) scaffo...
Osteogenic differentiation of two distinct subpopulations of human adipose-deri...
Rada, Tommaso; Santos, T. C.; Marques, A. P.; Correlo, V. M.; Frias, A. M.; Castro, António G.; Neves, N. M.; Gomes, Manuela E.; Reis, R. L.The first stem cells considered for the reconstruction of bone were bone marrow mesenchymal stem cells (BMSCs). Subsequently, cells with similar marker expression panel and differentiation potential were found in new sources of cells, such as adipose tissue. This source of stem cells has a promising future in tissue-engineering applications, considering the abundance of this tissue in the human body, the easy h...
Improved regeneration of mice full-thickness excisional wounds by human adipose...
Cerqueira, M. T.; Santos, T. C.; Pirraco, R. P.; Frias, A. M.; Martins, A. R.; Reis, R. L.; Marques, A. P.A totalidade dos resumos foram publicados em "J. Tissue Eng. Regen. Med. 2012; 6 (Suppl. 2) : 8–39."
Perivascular-like cells contribute to the stability of the vascular network of ...
Mendes, L. F.; Pirraco, R. P.; Szymczyk, Wojciech; Frias, A. M.; Santos, T. C.; Reis, R. L.; Marques, A. P.In recent years several studies have been supporting the existence of a close relationship in terms of function and progeny between Mesenchymal Stem Cells (MSCs) and Pericytes. This concept has opened new perspectives for the application of MSCs in Tissue Engineering (TE), with special interest for the pre-vascularization of cell dense constructs. In this work, cell sheet technology was used to create a scaffol...
Pericytes contribute to the stability of the vascular network of osteogenic tis...
Mendes, L. F.; Pirraco, R. P.; Szymczyk, Wojciech; Santos, T. C.; Frias, A. M.; Reis, R. L.; Marques, A. P.Despite the increased use of cell sheets for tissue engineering, the appli- cation of this new technology for thick tissue reconstruction is being limited by old barriers such as the need to create a pre-vascular net- work to ensure proper nutrient and oxygen supply in vivo. In the pres- ent work, we created a cell sheet-based construct by co-culturing bone marrow-derived osteogenic and pericyte-like cells with...
The use of ionic liquids in the processing of chitosan/silk hydrogels for biome...
Silva, S. S.; Santos, T. C.; Cerqueira, M. T.; Marques, A. P.; Reys, L. L.; Silva, Tiago H.; Caridade, S. G.; Mano, J. F.; Reis, R. L.Natural polymers are adequate renewable resources for the processability of well-defined architectures for several applications. Combinations of polysaccharides and proteins may mimic the naturally occurring environment of certain tissues. The main goal of this work renders the application of green chemistry principles, namely the use of ionic liquids (ILs) and biorenewable sources, such as chitosan (CHT) and s...
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