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Artefacts induced on c-type haem proteins by electrode surfaces
Sousa, Patrícia M. Paes de; Pauleta, Sofia R.; Gonçalves, M. Lurdes Simões; Pettigrew, Graham W.; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2011) 16:209–215 DOI 10.1007/s00775-010-0717-z ; In this work it is demonstrated that the characterization of c-type haem containing proteins by electrochemical techniques needs to be cautiously performed when using pyrolytic graphite electrodes. An altered form of the cytochromes, which has a redox potential 300 mV lower than that of the native state and displays peroxidatic activity, can b...
Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pan...
Sousa, P. M. Paes de; Pauleta, Sofia R.; Gonçalves, M. L. Simões; Pettigrew, Graham W.; Moura, Isabel; Moura, José J. G.J Biol Inorg Chem (2007) 12:691–698 DOI 10.1007/s00775-007-0219-9 ; This work reports the direct electrochemistry of Paracoccus pantotrophus pseudoazurin and the mediated catalysis of cytochrome c peroxidase from the same organism. The voltammetric behaviour was examined at a gold membrane electrode, and the studies were performed in the presence of calcium to enable the peroxidase activation. A formal reducti...
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
Moura, Isabel; Santos, Margarida M. Correia dos; Sousa, Patrícia M. P.; Gonçalves, M. Lurdes S.; Romão, M. João; Moura, José J. G.Eur. J. Biochem. 271, 1329–1338 (2004) ; This work reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2Fe-2S] centers and a molybdopterin cytosine dinucleotide cofactor. The voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and...
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