Encontrados 23 documentos, a visualizar página 1 de 3
Involvement of yeast HSP90 isoforms in response to stress and cell death induce...
Silva, Maria Alexandra; Marques, Belém Sampaio; Fernandes, Ângela Margarida Oliveira; Carreto, Laura; Rodrigues, Fernando José dos SantosAcetic acid-induced apoptosis in yeast is accompanied by an impairment of the general protein synthesis machinery, yet paradoxically also by the up-regulation of the two isoforms of the heat shock protein 90 (HSP90) chaperone family, Hsc82p and Hsp82p. Herein, we show that impairment of cap-dependent translation initiation induced by acetic acid is caused by the phosphorylation and inactivation of eIF2 alpha by...
Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...
Duarte, Ricardo Franco; Carrelo, Laura; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, Dorit; Cambon, BrigitteApresentação efetuada no Microbiotec2011, Braga, 2011 ; The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced ge...
Intra-strain phenotypic and genomic variability of the commercial Saccharomyces...
Duarte, Ricardo Franco; Carreto, Laura; Mendes, Inês; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, DoritThe use of commercial Saccharomyces cerevisiae wine strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest in large quantities. We showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes, not found among a control group of isolates that derived from clonal expansion of ...
Genetic diversity and population structure of Saccharomyces cerevisiae strains ...
Schuller, Dorit; Cardoso, Filipa; Sousa, Susana; Gomes, Paula; Gomes, Ana Catarina; Santos, Manuel A. S.; Casal, MargaridaWe herein evaluate intraspecific genetic diversity of fermentative vineyard-associated S. cerevisiae strains and evaluate relationships between grape varieties and geographical location on populational structures. From the musts obtained from 288 grape samples, collected from two wine regions (16 vineyards, nine grape varieties), 94 spontaneous fermentations were concluded and 2820 yeast isolates were obtained ...
Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing us...
Duarte, Ricardo Franco; Gomes, Ana Catarina; Santos, Manuel A. S.; Sousa, Bruno; Schuller, Dorit; Mendes, InêsAmplification of genomic sequences flanked by delta elements of retrotransposons TY1 and TY2 is a reliable method for characterization of Saccharomyces cerevisiae strains. The aim of this study is to evaluate the usefulness of microfluidic electrophoresis (Caliper LabChip®) to assess the factors that affect interlaboratory reproducibility of interdelta sequence typing for S. cerevisiae strain delimitation. We c...
Expression variability of co-regulated genes differentiates Saccharomyces cerev...
Carreto, Laura; Eiriz, Maria Francisca; Domingues, Inês; Schuller, Dorit; Moura, Gabriela; Santos, Manuel A. S.Background: Saccharomyces cerevisiae (Baker’s yeast) is found in diverse ecological niches and is characterized by high adaptive potential under challenging environments. In spite of recent advances on the study of yeast genome diversity, little is known about the underlying gene expression plasticity. In order to shed new light onto this biological question, we have compared transcriptome profiles of five envi...
Genetic characterization of commercial Saccharomyces cerevisiae isolates recove...
Duarte, Ricardo Franco; Carreto, Laura; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, DoritThe use of commercial S. cerevisiae wine strains as fermentation starters has been extensively generalized over the past two decades. Within our previous work we showed that such strains are disseminated from the winery and their permanence in nature induced genetic changes in comparison to the commercial “mother” strain. The objective of the present study was to evaluate genome variations among four isolates o...
Comparative genomics of commercial Saccharomyces cerevisiae isolates recovered ...
Duarte, Ricardo Franco; Carreto, Laura; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, DoritApresentação efetuada nas 36.ª Jornadas Portuguesas de Genética, Coimbra, 2011 ; The use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every year at the harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery and can ...
Microevolutionary changes of commercial Saccharomyces cerevisiae strains recove...
Duarte, Ricardo Franco; Carreto, Laura; Cambon, Brigitte; Dequin, Sylvie; Santos, Manuel A. S.; Casal, Margarida; Schuller, DoritThe use of commercial Saccharomyces cerevisiae wine yeast strains as fermentation starters has been extensively generalised over the past two decades. These strains are used by wineries every harvest time in large quantities. Within our previous work we showed that such strains are disseminated from the winery [1] and their permanence in nature induced genetic changes that were not found among a control group o...
A collection of indigenous saccharomyces cerevisiae strains from appellations o...
Vieira, E.; Neves, J. Drumonde; Gambon, Brigitte; Valero, Eva; Gomes, Ana Catarina; Sousa, Susana; Lima, Maria Teresa; Araújo, Isabel M.Apresentação efectuada nas "Jornadas Portuguesas de Genética, 35, Braga, 2010" ; The model organism Saccharomyces cerevisiae stands today at the forefront of molecular biology and functional analysis in genetics and genomics. However, understanding of the ecological, evolutionary and population genetic features that shaped the biology of this species is underscored by a wealth of knowledge on molecular and cel...
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