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Biological upgrading of wastes from the pulp and paper industry
Marques, Susana; Alves, Luís Manuel; Gírio, Francisco M.; Santos, J. A. L.; Roseiro, J. CarlosA process for biological upgrading of recycled paper sludge (RPS) was developed based on the enzymatic hydrolysis of major sludge components (cellulose and xylan) with commercial enzymes and fermentation of the resulting sugars into ethanol or lactic acid with adequate microbial strains. The process was implemented performing both steps sequentially (SHF) or simultaneously (SSF). Cellulosic and hemicellulosic f...
Optimization study of Escherichia coli TB1 cell disruption for cytochrome b5 re...
Belo, Isabel; Santos, J. A. L.; Cabral, J. M. S.; Mota, M.The recovery of a recombinant intracellular protein, cytochrome b5, from Escherichia coli TB1 cells was carried out by bead mill disintegration in a discontinuous smallscale instrument. This process was optimized by the use of experimental factorial design. Several parameters were studied: operating time, amount and size of beads, cellular suspension concentration, and presence of toluene and lysozyme. For the ...
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