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Effects of genetic polymorphisms at the growth hormone gene on milk yield in “S...
Marques, M. Rosário; Santos, Ingrid; Carolino, N.; Belo, Carmona; Renaville, Robert; Cravador, A.The five exons and the 5' and 3'-untranslated regions (5'-UTR and 3'-UTR) of the oGH gene were screened for mutations using PCR-single strand conformation polymorphism (PCR-SSCP) procedures in 523 Serra da Estrela ewes and were found to be highly polymorphic. The region extending across and between the GH2-N and GH2-Z copies was sequenced allowing the design of primers for the specific PCR amplification of each...
Single-Strand Conformation Polymorphism (SSCP) analysis of alfas1-casein, beta-...
Bonifácio, C.; Santos, Ingrid; Belo, Carmona; Cravador, A.The DNA from eighty goats belonging to the indigenous portuguese caprine breed Charnequeira, ecotype from Beira Baixa (Beiroa), was analysed. Single-strand conformation polymorphisms were identified at exon 9 and at exons 10-11 of the as1-casein gene and at exon 4 of the kcasein. The b-casein gene was found monomorphic at exon 7 in this population sample. The establishment of an association of some of these SSC...
Association between SSCPs at Algarvia goat GH and milk traits
Malveiro, Elisabete; Marques, P. X.; Santos, Ingrid; Belo, Carmona; Cravador, A.The DNA from one hundred eight goats belonging to the indigenous portuguese Algarvia breed was analysed. Single-strand conformation polymorphismÕs (SSCP) were identified at the five exons of the goat Growth Hormone (gGH) gene. Two conformational patterns were found in each of exons 1 and 2, four in exon 3, seven in exon 4 and five in exon 5. The establishment of an association of these SSCP patterns with milk, ...
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