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The use of MALDI-TOF ICMS as an alternative tool for Trichophyton rubrum identi...
Pereira, Leonel; Dias, Nicole; Santos, Cledir; Lima, NelsonIn this study, the potential of matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF ICMS) was investigated for the identification of clinical isolates. The isolates were analyzed at the species and strain level,
Diversity of Penicillium in soil of Caatinga and Atlantic Forest areas of Perna...
Cruz, Roberta; Santos, Cledir; Lima, Juliana Silva de; Moreira, Keila; Motta, Cristina SouzaCaatinga is characterised as being a unique semi-arid biome only found in Brazil. It is characterised mainly for its soil poor in mineral and organic nutrients, and low water activity. On the other hand, Atlantic Forest is mainly characterised by its nutrient-rich soil, and its high water activity. Fungi are important constituents of both biomes. Among the fungi frequently isolated from soil of both Caatinga an...
Effect of different carbon sources on decolourisation of an industrial textile ...
Ottoni, C. A.; Lima, Luís; Santos, Cledir; Lima, NelsonWhite-rot fungal strains of Trametes versicolor and Phanerochaete chrysosporium were selected to study the decolourisation of the textile dye, Reactive Black 5, under alkaline–saline conditions. Free and immobilised T. versicolor cells showed 100 % decolourisation in the growth medium supplemented with 15 g l-1 NaCl, pH 9.5 at 30 C in liquid batch culture. Continuous culture experiments were performed in a fix...
Structural diversity of aspergillus (section nigri) spores
Simões, Marta Filipa; Santos, Cledir; Lima, NelsonThe taxonomy of fungal species, similar to that of many other microorganisms, suffers frequent revisions due to the discovery of new species and to the development and gathering of characterization data and morphological information. Morpho-taxonomy helps in the identification of many species. This work presents the macro, micro-morphological, and spectral mass analyses for phenotypic characterization of 13 spe...
Evaluation of inhibitory effects of Trichoderma asperellum metabolites on mycel...
Filho, Magno Rodrigues de Carvalho; Craveiro, Saluana Rocha; Martinhs, Irene; Lima, Nelson; Santos, Cledir; Miller, Robert Neil GerardCommon bean (Phaseolus vulgaris L.) is an economically important food commodity in Brazil. However, it can suffer serious damage by white mould disease caused by the pathogen Sclerotinia sclerotiorum. Species of the genus Trichoderma can act as biocontrol agents against of this pathogen. This study describes the identification based on morphological, MALDI-TOF MS and molecular analysis of these 29 Trichoderma s...
Identification and phylogeny of Trichoderma species by MALDI-TOF analysis and c...
Filho, Magno Rodrigues de Carvalho; Craveiro, Saluana Rocha; Martinhs, Irene; Lima, Nelson; Santos, Cledir; Miller, Robert Neil GerardCommon bean (Phaseolus vulgaris L.) is an economically important food commodity in Brazil. However, it can suffer serious damage by white mould disease caused by the pathogen Sclerotinia sclerotiorum. Species of the genus Trichoderma can act as biocontrol agents against of this pathogen. This study describes the identification based on morphological, MALDI-TOF MS and molecular analysis of these 29 Trichoderma s...
MALDI-TOF MS: improved methods for the identification/characterisation and auth...
Santos, Cledir; Rodrigues, Paula; Venâncio, Armando; Lima, NelsonThe identification of species is an important goal in taxonomic mycology. Information about each fungus (e.g. morphological description, physiological and biochemical properties, ecological roles, and societal risks or benefits) is key element in this process. Identifications can be a long and seemingly never-ending process with frequent revisions of the taxonomic schemes. These changes make identifications eve...
Taxonomy of Aspergillus: integration of methods for new and revisited species i...
Rodrigues, Paula; Soares, Célia; Santos, Cledir; Venâncio, Armando; Lima, NelsonAspergillus is a large genus and, as with fungi in general, its taxonomy is complex and ever evolving. Section Flavi is one of the most significant sections of the genus, for it comprises two distinct groups of species with great interest to food industry: one group includes the aflatoxigenic species, which cause serious problems in agricultural commodities, and the other includes the non-aflatoxigenic species ...
MALDI-TOF ICMS as a modern approach to identify potential aflatoxigenic fungi
Rodrigues, Paula; Santos, Cledir; Venâncio, Armando; Lima, NelsonThe Aspergillus section Flavi is among the best studied fungi, having different commercial applications, but also causing biodeterioration of commodities and food spoilage. Fungi from this Section are also responsible for the production of highly toxic secondary metabolites – the aflatoxins. They are morphologically and genetically very similar, and can be difficult to differentiate by both cultural and molecul...
Portuguese isolates of Aspergillus section Flavi unraveled by the calmodulin gene
Rodrigues, Paula; Torres, Aldara; Nunes, Florbela; Leirós, Catarina; Santos, Cledir; Venâncio, Armando; Lima, NelsonAspergillus is a large genus, with a complex and ever evolving taxonomy. Section Flavi is one of the most significant sections in the genus. Taxonomy and species identification is subject of great interest for scientists aiming to clarify the species concept and limits within the section. Furthermore, this section comprises both toxigenic and non-toxigenic species/strains, with great interest to biotechnology a...
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