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RpoB polymorphisms in Mycobacterium tuberculosis complex from a population in G...

The global tuberculosis (TB) threat is diffi cult to evaluate in countries with scarce laboratory facilities where little is known about the genetic basis of drug resistance, including the rpoB RRDR (rifampicin resistance determining region) of the Mycobacterium tuberculosis complex (MTC). The present study aimed at performing a preliminary evaluation of rpoB polymorphisms in a DNA set of MTC isolates from Guin...

Staphylococcus aureus reservoirs and transmission routes in a Portuguese Neonat...

Although Staphylococcus aureus is a major cause of outbreaks in neonatal intensive care units (NICUs), there are no studies on the epidemiology of S. aureus isolates responsible for infection in Portuguese NICUs. Between July 2005 and December 2007, a total of 54 methicillin susceptible S. aureus (MSSA) isolates were recovered from 16 infected infants, parents, health care workers (HCWs), and the environment in...

Bactérias vs antimicrobianos: who's the winner?

High prevalence of ST121 in community-associated methicillin-susceptible Staphy...

In order to evaluate the incidence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) in Portugal, we analyzed a collection of 38 S. aureus isolates recovered from 30 children attending the pediatric emergency department of a central hospital in Lisbon due to skin and soft tissue infections. Molecular characterization identified seven clonal lineages among the 35 methicillin-susceptib...

Contribution of spoligotyping to the characterization of the population structu...

Tuberculosis is a major health problem in Portugal. To begin characterizing the population structure of Mycobacterium tuberculosis, spoligotyping was used for the systematic typing, through consecutive sampling, of patient isolates from the Amadora-Sintra area of Greater Lisbon. Distribution amongst major spoligotype families, including the Latin American Mediterranean (LAM), T, Haarlem and Beijing, was compare...

Novos dados sobre os Spoligotypes de estirpes do complexo Mycobacterium tubercu...

O presente estudo populacional, que decorreu entre 1999 e 2003, foi baseado na utilização do Spoligotyping na genotipagem de 452 isolados do complexo Mycobacterium tuberculosis de doentes com tuberculose internados no Hospital Fernando Fonseca. Spoligotypes foram identificados como shared types (ST) recorrendo a uma base de dados internacional. Onze ST raros, não identificados na base de dados, acomodaram 8,4% ...

Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diag...

The differential diagnosis of Mycobacterium bovis is important in the control of transmission to the human population and also for treatment since M. bovis is naturally resistant to pyrazinamide. Eleven clinical isolates from the Fernando Fonseca Hospital with Spoligotypes indicative of M. bovis, through the absence of spacers 39-43 but that also counted with the absence of spacer 38, were analyzed. For the ide...

Direct application of the INNO-LiPA Rif.TB line-probe assay for rapid identific...

The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and the detection of rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area of high incidence of multidrug-resistant tuberculosis (MDR-TB). The sensitivity when compared to conventional identification/culture methods was 82.2%, and the specificity was 66.7%; the sensit...

Identificação molecular pelo método de Spoligotyping de estirpes do complexo My...

Spoligotyping was used in the genotyping of 219 isolates of the Mycobacterium tuberculosis complex, from patients of the Hospital Fernando Fonseca. This technique, based on PCR methodology, analyses a region of the chromosome specific of the Mycobacterium tuberculosis complex, the DR locus (Direct Repeat). With the aid of an international database, we showed that the predominant Spoligotypes belonged to the LAM...