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Genotipagem de Candida parapsilosis com marcadores de DNA microssatélite: uma f...

Candida bracarensis virulence factors

Isolates from hospital environments are the most virulent of the Candida paraps...

Background: Candida parapsilosis is frequently isolated from hospital environments, like air and surfaces, and causes serious nosocomial infections. Molecular studies provided evidence of great genetic diversity within the C. parapsilosis species complex but, despite their growing importance as pathogens, little is known about their potential to cause disease, particularly their interactions with phagocytes. In...

Tear film stability and immunoglobulin a variation during the day

Tear film stability and immunoglobulin A variation during the day A. Puinhas1, M. Lira1, S Franco1 and M P Sampaio2 1.Centre of Physics (CFUM), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. 2. Centre of Molecular and environmental Biology (CBMA), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. . Currently, there are more than 100 million contact lenses (CL) wearers worldwi...

Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spe...

Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensi...

New polymorphic microsatellite markers able to distinguish among Candida paraps...

Among the Candida species causing bloodstream infections, Candida parapsilosis is one of the most frequently isolated. The objective of the present work was the identification of new microsatellite loci able to distinguish among C. parapsilosis isolates. DNA sequences with trinucleotide repeats were selected from the C. parapsilosis genome database. PCR primer sets flanking the microsatellite repeats were desig...

Virulence attenuation of Candida albicans genetic variants isolated from a pati...

The incidence of Candida albicans infections and the relapse episodes after antifungal treatment have increased in recent decades. Recurrences are mainly due to the persistence of the original infecting strain that may present genetic and genomic rearrangements during interaction with the host, reflecting strain adaptation. In this study, four isolates recovered from a patient during recurrent candidemia episod...

Rheological and structural characterization of gels from whey protein hydrolysa...

The gelling ability of whey proteins can be changed by limited hydrolysis and by the addition of other components such as polysaccharides. In this work the effect of the concentration of locust bean gum (LBG) on the heat-set gelation of aqueous whey protein hydrolysates (10% w/w) from pepsin and trypsin was assessed at pH 7.0. Whey protein concentrate (WPC) mild hydrolysis (up to 2.5% in the case of pepsin and ...

Dynein and Mast/Orbit/CLASP have antagonistic roles in regulating kinetochore-m...

Establishment and maintenance of the mitotic spindle requires the balanced activity of microtubule-associated proteins and motors. In this study we have addressed how the microtubule plus-end tracking protein Mast/Orbit/CLASP and cytoplasmic dynein regulate this process in Drosophila melanogaster embryos and S2 cells. We show that Mast accumulates at kinetochores early in mitosis, which is followed by a polewar...

Reading literature today: a study of E. M. Forster s and George Orwell s fiction

Tese de doutoramento em Estudos de Cultura (Cultura Inglesa), apresentada à Universidade de Lisboa através da Faculdade de Letras, 2008