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QUANTIFICAÇÃO DA PROTEÍNA CRY1AB EM FOLHAS, CAULES E GRÃOS DE DOIS HÍBRIDOS DE ...

Balieiro Neto, Geraldo; Cividanes, Terezinha; Branco, Roberto; Félix, M. Rosário; Rei, Fernando; Nogueira, José

RESUMO: Procedeu-se à avaliação do nível de infestação e respectivos danos associados à presençadas lagartas Spodoptera frugiperda e Helicoverpa zea, em dois milhos híbridos geneticamentemodificados (OGM), Dekalb DKB390 e Agroceres AG8088, que expressam a proteína Cry1Ab. Foi estabelecido em blocos casualizados, com dois fatores (hibrido e gene cry1Ab), sendo utilizadascomo controles os mesmos híbridos sem a pr...


First characterization of infectious cDNA clones of Olive mild mosaic virus

Cardoso, Joana M.S.; Félix, M. Rosário; Clara, M. Ivone; Oliveira, Solange

Full-length cDNA clones of an Olive mild mosaic virus (OMMV) isolate were constructed in order to find infectious cDNA clones. The sequencing of three individual full-length clones revealed some differences between them. In vitro transcription of these clones was performed and the effect of spontaneous mutations in the biological behaviour of the in vitro transcripts was evaluated by symptomatology, RNA accumul...


Biology and molecular characterization of necroviruses

Félix, M. Rosário; Varanda, Carla M.R.; Clara, M. Ivone E.

Three necroviruses, family Tombusviridae, are known to infect Olea europaea L., Olive latent virus 1 (OLV-1), Olive mild mosaic virus (OMMV) and Tobacco necrosis virus D (TNV-D), often asymptomatically. OLV-1 has a wide geographical distribution whereas that of OMMVand TNV-D is not known as their discovery is recent, dating from 2005 and 2009, respectively. OMMV has received special attention because of its ori...


Evidence of olive mild mosaic virus transmission by Olpidium brassicae

Varanda, Carla M. R.; Silva, Marta S. M. R.; Félix, M. Rosário; Clara, M. Ivone

Transmission of three strains of OMMV by an Olpidium sp. was evaluated and compared. The three strains were 1) an OMMV wild type (WT) recovered from olive trees, 2) an OMMV variant (L11) obtained after 15 serial passages of single local lesions induced in Chenopodium murale plants, and 3) a construct OMMV/OMMVL11 in which the coat protein (CP) gene replaced that of the wild type. A single-sporangial culture der...


Relação Custo Benefício na Produção de Silagem com Milho Bt

Balieiro, Geraldo N.; Branco, Roberto B.F.; Cividanes, Terezinha M.S.; Nogueira, José R.; Félix, M. Rosário; Roma Junior, Luiz C.; Bueno, Mauro S.

Eficiência e escala de produção são imprescindíveis para obtenção de retornos financeiros compensatórios na atividade pecuária. O aumento da produtividade demanda maior produção de energia digestível por área, a fim de suprir plenamente as exigências nutricionais dos animais ao longo do ano de forma menos dispendiosa. O custo com alimentação dos animais se eleva no período seco do ano devido à redução no cresci...


Multiplex RT-PCR for detection and identification of three necroviruses that in...

Varanda, Carla; Cardoso, Joana M.S.; Félix, M. Rosário; Oliveira, Solange; Clara, M.Ivone

An optimized multiplex RT-PCR assay was developed to discriminate three necrovirus (Olive latent virus 1 (OLV-1), Tobacco necrosis virus D (TNV-D) and Olive mild mosaic virus (OMMV)) that infect olive trees. An olive orchard consisting of 54 trees of cv. "Galega vulgar" in the south of Portugal was surveyed. dsRNA fraction was used as template and revealed the 3 viruses, singly or in multiple infections, presen...


Complete genome sequence of a Tobacco necrosis virus D isolate from olive trees

Cardoso, Joana S.; Félix, M.Rosário; Clara, M.Ivone; Oliveira, Solange

The first complete genome sequence of an isolate of Tobacco necrosis virus D (TNV-D; genus Necrovirus,family Tombusviridae) was that of a bean isolate from England. The complete nucleotide sequence of another TNV-D isolate, an Hungarian isolate from tobacco plants (TNV-DH), revealed a similar genomic organization. The single-stranded positive-sense RNA genome of TNV-D is about 3.8 kb in size and contains six op...


Biological and Molecular Characterization of Olive latent virus 1

Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.

Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...


Biological and Molecular Characterization of Olive latent virus 1

Félix, M. Rosário; Cardoso, Joana M.S.; Oliveira, Solange; Clara, M. Ivone E.

Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides ...


Monoclonal antibody to Olive latent virus 1

Custódio, Ana I.; Cardoso, Fernando H.; Félix, M. Rosário; Clara, M. Ivone; Novo, Carlos

Olive is a crop of high economic importance in Portugal. Its susceptibility to virus infections justifies research leading to accurate virus detection. Two necroviruses mechanically transmissible to herbaceous indicator plants have been isolated from symptomatic olive trees: Olive latent virus 1 (OLV-1) and Tobacco necrosis virus D (TNV-D). OLV-1 was also isolated from citrus trees in Turkey and from tulips in ...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia