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Lack of bursting electrical activity and intracellular calcium oscillations in ...
Antunes, Célia M.; Salgado, António P.; Rosário, Luís M.; Santos, Rosa M.http://www.sciencedirect.com/science/article/B6T5Y-49PK0KM-M1/1/868808b020f37b7932fbbb7f28b3f46b
Glucose-mediated Ca2+ signalling in single clonal insulin-secreting cells: evid...
Salgado, António P.; Santos, Rosa M.; Fernandes, Ana P.; Tomé, Angelo R.; Flatt, Peter R.; Rosário, Luís M.Using clonal insulin-secreting BRIN-BD11 cells, we have assessed whether the graded response of the whole cell population to glucose can be accounted for by a dose-dependent recruitment of individual cells, an amplification of the response of the recruited cells or both. Cytosolic free Ca2+ concentration ([Ca2+]i) is an established index of [beta]-cell function. We used fura-2 microfluorescence techniques to as...
Differential patterns of glucose-induced electrical activity and intracellular ...
Antunes, Célia M.; Salgado, António P.; Rosário, Luís M.; Santos, Rosa M.Although isolated rat islets are widely used to study in vitro insulin secretion and the underlying metabolic and ionic processes, knowledge on the properties of glucose-induced electrical activity (GIEA), a key step in glucose-response coupling, has been gathered almost exclusively from microdissected mouse islets. Using a modified intracellular recording technique, we have now compared the patterns of GIEA in...
Multiphasic action of glucose and alpha-ketoisocaproic acid on the cytosolic pH...
Salgado, António P.; Silva, Amélia M.; Santos, Rosa M.; Rosário, Luís M.Glucose stimulation raises the pHi of pancreatic beta-cells, but the underlying mechanisms are not well understood. We have now investigated the acute effects of metabolizable (glucose and the mitochondrial substrate alpha-ketoisocaproic acid, KIC) and nonmetabolizable (high K+ and the K-ATP channel blocker tolbutamide) insulin secretagogues on the pHi of pancreatic beta-cells isolated from normal mice, as asse...
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