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Behaviour of human erythrocyte aggregation in presence of autologous lipoproteins

The aim of this work was to evaluate in vitro the effect of autologous plasma lipoprotein subfractions on erythrocyte tendency to aggregate. Aliquots of human blood samples were enriched or not (control) with their own HDL-C, LDL-C, or VLDL-C fractions obtained from the same batch by density gradient ultracentrifugation. Plasma osmolality and erythrocyte aggregation index (EAI) were determined. Blood aliquots e...

Behaviour of human erythrocyte aggregation in presence of autologous lipoproteins

Copyright © 2012 C. Saldanha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ; The aim of this work was to evaluate in vitro the effect of autologous plasma lipoprotein subfractions on erythrocyte tendency to aggregate. Aliquots of human b...

Evidence that the degree of band 3 phosphorylation modulates human erythrocytes...

© 2011 – IOS Press and the authors. All rights reserved ; Recent evidence has shown that plasma fibrinogen, a major cardiovascular risk factor, interacts with the erythrocyte membrane and acts to influence blood flow via erythrocyte nitric oxide (NO) modulation. In the present pioneer in-vitro study, whole blood samples were harvested from healthy subjects and aliquots were incubated in the absence (control a...

Erythrocyte as a link between basic and clinical research

© 2011 – IOS Press and the authors ; We review the major hemorheological experimental studies that show the erythrocyte aggregation as a link between basic and clinical research. The results of the clinical cross-sectional and longitudinal studies presented here will highlight the possible association between erythrocyte aggregation and plasma fibrinogen. Basic studies conducted in vitro are also mentioned as ...

Dithiothreitol revisited in red cells : a new head for an old hat

© 2010 – IOS Press and the authors. All rights reserved ; In the present article the authors make an approach over the applications of dithiothreitol (DTT) in its different clinicallaboratory, potential and up-to-date sources. Dithiothreitol is a chemical reagent with a wide actuation spectrum not only from a laboratorial view but also from a therapeutic standpoint, more clinical and practical. DTT (i) is freq...

Erythrocyte senescence: lipo-protein interactions of the erythrocyte membrane.

Red cell abnormalities in a kindred with an uncommon form of hereditary spheroc...

The effect of angiography with disodium fluorescein on the hemorheologic parame...

Chorioretinal angiography with fluorescein is an auxiliary method widely used in Ophthalmology which enables us to study the blood/humour and blood/retina/choroid interface. The presence of a strange compound in circulation may interfere with blood flow homeostasis. With the aim of studying fluorescein influence in blood rheology, blood samples were drawn before, immediately after and 30 minutes after fluoresce...

Evaluation of blood esterases in Alzheimer's disease.

The aim of this work was to evaluate a possible correlation between erythrocyte acetylcholinesterase activity (AChE) and membrane fluidity expressed by fluorescence polarization of--1,6--diphenyl 1,3,5--hexatriene (DPH). Blood samples of 34 Alzheimer's patients (18F and 16M) were obtained and haemoglobin concentration, haematocrit, plasma (butyrylcholinesterase, BuChE) and erythrocyte (AChE) esterase activities...

Evaluation of interests, experiences and chemistry knowledge among students adm...

Selected medical students for undergraduate medical courses in Portugal exhibit each year substantial deficiencies in chemistry. The group of students admitted to the Faculty of Medicine of Lisbon in 1989/1990 were selected by admission criteria apparently more restrictive than before. The aim of this study was to evaluate the quality of the theoretical knowledge in chemistry showed by those students before lea...