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The action of selected isothiocyanates on bacterial biofilm prevention and control
Borges, A.; Simões, Lúcia C.; Saavedra, Maria J.; Simões, M.The activity of two selected isothiocyanates (ITCs), allylisothiocyanate (AITC) and 2-phenylethy-lisothiocyanate (PEITC) was evaluated on the prevention and control of biofilms formed by Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes. In addition, the effect of ITCs was also tested on planktonic cell susceptibility, bacterial motility and adhesion. Biofilm prevention ...
Persister cells in a biofilm treated with a biocide
Simões, Lúcia C.; Lemos, Madalena; Pereira, Ana M.; Abreu, Ana C.; Saavedra, Maria J.; Simões, M.This study investigated the physiology and behaviour following treatment with ortho-phthalaldehyde (OPA), of Pseudomonas fluorescens in both the planktonic and sessile states. Steady-state biofilms and planktonic cells were collected from a bioreactor and their extracellular polymeric substances (EPS) were extracted using a method that did not destroy the cells. Cell structure and physiology after EPS extractio...
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