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Bioelectrochemical investigations of aryl-alcohol oxidase from Pleurotus eryngii
Munteanu, Florentina-Daniela; Ferreira, Patrícia; Ruiz-Duenas, Francisco J.; Martínez, Angel T.; Paulo, Artur CavacoArticle in Press ; Aryl-alcohol oxidase (AAO) electrochemistry studies, using graphite-modified electrodes, are presented for the first time herein. The increase in current upon injection of the analyzed substrate was shown to be approximated by Michaelis–Menten type dependence. The calculated kinetic constants were used to characterize the native (non-mutated) recombinant AAO expressed in Escherichia coli, as...
Kinetics of direct and substrate-mediated electron transfer of versatile peroxi...
Munteanu, Florentina-Daniela; Ruiz-Duenas, Francisco J.; T. Martinez, Angel; Paulo, Artur CavacoElectron transfer (ET) of versatile peroxidase (VP) was studied in the bioelectrocatalytic reduction reaction of H2O2 at peroxidase- modified graphite electrodes to obtain additional information on the kinetic characteristics of this novel ligninolytic peroxidase. Rotating disk electrodes (RDE) experiments were performed at 0 V (vs. SCE) in two different buffers (tartrate buffer, pH 5.0; and citrate buffer, pH ...
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