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Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
Pinto, João Ricardo; Amaral, A. L.; Ferreira, E. C.; Mota, M.; Vilanova, Manuel; Ruel, Katia; Gama, F. M.Background Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC). The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results For a CBD-FITC concentration of 60 mg/L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Wh...
Data: 2008
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Origem: RepositóriUM - Universidade do Minho
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