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Honey bees (Hymenoptera: Apidae) of african origin exist in non-africanized are...
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Schiff, Nathan M.; Kandemir, Irfan; Patton, John C.Descendents of Apis mellifera scutellata Lepeletier (Hymenoptera: Apidae) (the Africanized honey bee) arrived in the United States in 1990. Whether this was the Þrst introduction is uncertain. A survey of feral honey bees from non-Africanized areas of the southern United States revealed three colonies (from Georgia, Texas, and New Mexico) with a diagnostic African mitochondrial DNA cytochrome b/BglII fragment p...
Africanization in the United States: Replacement of Feral European Honeybees (A...
Pinto, M. Alice; Rubink, William L.; Patton, John C.; Coulson, Robert N.; Johnston, J. SpencerThe expansion of Africanized honeybees from South America to the southwestern United States in 50 years is considered one of the most spectacular biological invasions yet documented. In the American tropics, it has been shown that during their expansion Africanized honeybees have low levels of introgressed alleles from resident European populations. In the United States, it has been speculated, but not shown, ...
Feral honey bees in pine forest landscapes of east Texas
Coulson, Robert N.; Pinto, M. Alice; Tchakerian, Maria D.; Baum, Kristen A.; Rubink, William L.; Johnston, J. SpencerIn 1990 the Africanized honey bee, a descendent of Apis mellifera scutellata, was identified in south Texas [Hunter, L.A., Jackman, J.A., Sugden,E.A., 1992.Detection records of Africanized honey bees inTexas during 1990, 1991 and 1992. Southwestern Entomol. 18, 79–89]. The potential impact of this immigrant on feral and managed colonies was the subject of considerable speculation. The goal of this study was to ...
Spatial and temporal distribution and nest site characteristics of feral honey ...
Baum, Kristen A.; Rubink, William L.; Pinto, M. Alice; Coulson, Robert N.We evaluated the distribution and abundance of feral honey bee, Apis mellifera L., colonies in a coastal prairie landscape by examining nest site characteristics, population trends, and spatial and temporal patterns in cavity use. The colony densities of up to 12.5 colonies per km2 were the highest reported in the literature for an area including both suitable and unsuitable patches of nesting habitat. The meas...
Temporal pattern of africanization in a feral honeybee population from Texas in...
Pinto, M. Alice; Rubink, William L.; Coulson, Robert N.; Patton, John C.; Johnston, J. SpencerThe invasion of Africanized honeybees (Apis mellifera L.) in the Americas provides a window of opportunity to study the dynamics of secondary contact of subspecies of bees that evolved in allopatry in ecologically distinctive habitats of the Old World. We report here the results of an 11-year mitochondrial DNA survey of a feral honeybee population from southern United States (Texas). The mitochondrial haplotype...
Evidence of African honey bees mitotypes in the southern United States prior to...
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.The standard protocol for assessing honey bees with a mitotype derived from an African origin is the amplification of a segment of cytochrome b and subsequent digestion of the amplified fragment with the restriction enzyme Bgl II. A previous survey of 451pre-Africanization honey bees from the southern U. S. revealed three bees with restriction pattern consistent with an African mitotype. To confirm these bees t...
Feral honey bees in pine forest landscapes of East Texas
Coulson, Robert N.; Pinto, M. Alice; Tchakerian, Maria D.; Baum, Kristen A.; Rubink, William L.; Johnston, J. SpencerThe goal of this study was to investigate the diversity of feral honey bee races in pine forest landscapes of east Texas, subsequent to immigration of Africanized honey bees, Apis mellifera scutellata. The specific objectives were (i) to assess the immigration of A. m. scutellata into east Texas pine forest landscapes and (ii) to evaluate the suitability of the pine forest landscape to feral honey bees. This me...
Identification of africanized honey bee (Hymenoptera: apidae) mitochondrial DNA...
Pinto, M. Alice; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C.; Sheppard, Walter S.Polymerase chain reaction (PCR)-ampliÞed mitochondrialDNA(mtDNA)assays have been used in studies of the Africanization process in neotropical feral and managed honey bee populations. The approach has been adopted, in conjunction with morphometric analysis, to identify Africanized bees for regulatory purposes in the United States such as in California. In this study, 211 Old World colonies, representing all know...
Long term preservation of DNA from honey bees (Apis mellifera) collected in aer...
Rubink, William L.; Murray, K. D.; Baum, Kristen A.; Pinto, M. AliceThis study examines the preservation of nuclear and mitochondrial DNA from honey bee (Apis mellifera L.) specimens which were first kept in propylene glycol-based antifreeze under various conditions, and then stored long-term, refrigerated in 95% ethanol. Two sets of bees were subjected to the propylene glycol treatment, then ethanol storage. One set consisted of bees captured in the field in propylene glycol-c...
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