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New water-soluble amine-reactive reagents for labeling microbial cells: applica...
Martins, Sérgio; Rosado, Tânia; Caldeira, Ana; Pereira, AntónioMural paintings are continuously exposed to physical, chemical and biological degradation, however, among the biological agents that cause deterioration, microorganisms are of critical importance, due to their metabolic versatility and flexibility, and their ability to tolerate unfavourable conditions. Thereby, microorganisms can cause damages in mural paintings surfaces through a variety of mechanisms, includi...
Evaluation of anti-microbial potential of hydroxycoumarins
Fernandes, Cátia; Martins, Sérgio; Rosado, Tânia; Caldeira, Ana; Pereira, AntónioCoumarins comprise a very large class of substances found in plants and are made of fused benzene and α-pyrone rings. More than 1300 coumarins have been identified, principally as secondary metabolites in green plants but also in fungi and bacteria. Coumarin and its derivatives are considered nowadays an important class of organic compounds due to their wide spectrum of biological activities and therapeutic app...
Evaluation of anti-microbial potential of 3-ethenylcoumarins and their precursors
Ramos, João; Martins, Sérgio; Rosado, Tânia; Caldeira, Ana; Pereira, AntónioCoumarins, classified as benzo-α-pyrones, are part of a large class of secondary phenolic metabolites derivatived from cinnamic acid, widely distributed in the plant kingdom but can also be found in fungi and bacteria. Today there are more than 1300 identified structures. In some plants they are mainly accumulated in the leaves, inhibiting the growth of pathogenic fungi and repelles insects. Coumarins are nowad...
ENZYMATIC MONITORIZATION OF MURAL PAINTINGS BIODETERIORATION
Rosado, Tânia; Martins, M. Rosário; Pires, Margarida; Mirão, José; Candeias, A; Caldeira, A TeresaBiodeterioration of mural paintings by microorganisms is an important research field that needs novel approaches to fully understand the mechanisms and effects. In this work, the presence of microorganisms and their biological activity were investigated by extra and intracellular enzymatic monitorization. The enzymes arylsulphatase, β-glucosidase, dehydrogenase and phosphatase were used as biomarkers of the mic...
Enzymatic systems as biomarkers of cultural heritage decay: A new approach to a...
Pires, Margarida; Rosado, Tânia; Mirão, José; Candeias, António; Martins, M. Rosário; Caldeira, A. TeresaCultural Heritage decay is a serious problem with social and economic impact. Mural painting is one of the artistic expressions widely represented in Portugal, which over the time has suffered numerous forms of deterioration. Heritage decay depends on the physical properties and chemical nature of the materials, several environmental parameters and biological agents. The most harmful organisms involved in biode...
Enzymatic monitorization of mural paintings biodeterioration
Rosado, Tânia; Pires, Margarida; Mirão, José; Candeias, António; Martins, M. Rosário; Caldeira, A. TeresaMural paintings is not only a form of art but also a way of learn more about our ancestral traditions - that´s why it´s so important to protected it from biodegradation. There are several factors that affect this artworks, such as environmental conditions, chemical nature of the substrate and biological agents.
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