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Expression and function of K(ATP) channels in normal and osteoarthritic human c...

Rufino, AT; Rosa, SC; Judas, F; Mobasheri, A; Lopes, MC; Mendes, AF

ATP-sensitive potassium [K(ATP)] channels sense intracellular ATP/ADP levels, being essential components of a glucose-sensing apparatus in various cells that couples glucose metabolism, intracellular ATP/ADP levels and membrane potential. These channels are present in human chondrocytes, but their subunit composition and functions are unknown. This study aimed at elucidating the subunit composition of K(ATP) ch...



Role of glucose as a modulator of anabolic and catabolic gene expression in nor...

Rosa, SC; Rufino, AT; Judas, F; Tenreiro, CM; Lopes, MC; Mendes, AF

Cartilage matrix homeostasis involves a dynamic balance between numerous signals that modulate chondrocyte functions. This study aimed at elucidating the role of the extracellular glucose concentration in modulating anabolic and catabolic gene expression in normal and osteoarthritic (OA) human chondrocytes and its ability to modify the gene expression responses induced by pro-anabolic stimuli, namely Transformi...


Expression and function of the insulin receptor in normal and osteoarthritic hu...

Rosa, SC; Rufino, AT; Judas, F; Tenreiro, C; Lopes, MC; Mendes, AF

OBJECTIVE: Chondrocytes respond to insulin, but the presence and role of the specific high affinity insulin receptor (InsR) has never been demonstrated. This study determined whether human chondrocytes express the InsR and compared its abundance and function in normal and osteoarthritis (OA) human chondrocytes. DESIGN: Cartilage sections were immunostained for detection of the InsR. Non-proliferating chondrocy...


Assessment of strategies to increase chondrocyte viability in cryopreserved hum...

Rosa, SC; Gonçalves, J; Judas, F; Lopes, C; Mendes, AF

OBJECTIVE: Allogeneic cartilage is used to repair damaged areas of articular cartilage, requiring the presence of living chondrocytes. So far, no preservation method can effectively meet that purpose. Identification of more effective cryoprotective agents (CPAs) can contribute to this goal. The aim of this study was to determine whether the glycosylated hydroquinone, arbutin, alone or in combination with low co...


Impaired glucose transporter-1 degradation and increased glucose transport and ...

Rosa, SC; Gonçalves, J; Judas, F; Mobasheri, A; Lopes, C; Mendes, AF

INTRODUCTION: Disorders that affect glucose metabolism, namely diabetes mellitus (DM), may favor the development and/or progression of osteoarthritis (OA). Thus far, little is known regarding the ability of chondrocytes to adjust to variations in the extracellular glucose concentration, resulting from hypoglycemia and hyperglycemia episodes, and so, to avoid deleterious effects resulting from deprivation or int...


Nitric oxide synthase isoforms and NF-kappaB activity in normal and osteoarthri...

Rosa, SC; Judas, F; Lopes, MC; Mendes, AF

To elucidate the role of endogenous inducible nitric oxide (NO) on the regulation of NF-kappaB activity in human chondrocytes, we evaluated (i) the pattern of expression of the neuronal (nNOS) and inducible (iNOS) NO synthase isoforms and the basal NF-kappaB activity in normal and osteoarthritic (OA) human chondrocytes, (ii) the role of cytokines and growth factors in modulating the protein levels of the two NO...


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    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia