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Helicobacter pylori - Associated Dyspepsia in Paediatrics

Helicobacter pylori ubiquitously infects the human gastric mucosa since time immemorial,predictably before the man’s diaspora out of East Africa around 58,000 years ago [1].Colonization may have been somehow beneficial for human carriers, allowing the coevolutionof this gram-negative bacterium and its host over the centuries. Yet, at leastnowadays [2], this may not be a peaceful association, with infection almo...

The ulcerogenic profile of Helicobacter pylori paediatric strains associated wi...

Helicobacter pylori infection is the major cause of paediatric peptic ulcer disease (PUD). In children with no other aetiology for the disease, this rare event occurs shortly after infection, presuming a still poorly understood higher susceptibility of the patient and highlighting the virulence of the implicated strain. Recently, we showed that the enhanced virulence of a group of paediatric ulcerogenic-strains...

Screening of Prophage Sequences Among Helicobacter Pylori

Until recently, Helicobacter pylori was considered a bacterium without prophages. The presence of an incomplete prophage sequence in strain B38 and a complete prophage sequence in strain B45 showed otherwise. Using a PCR strategy, based on degenerated primers designed after aligning bacteriophage integrase genes from H. pylori strains B38 and B45, and H. acinonychis prophage II, we found that integrase sequence...

Ulcerogenic Helicobacter pylori Strains Isolated from Children: A Contribution ...

Infection with Helicobacter pylori is the major cause for the development of peptic ulcer disease (PUD). In children, with no other etiology for the disease, this rare event occurs shortly after infection. In these young patients, habits of smoking, diet, consumption of alcohol and non-steroid anti-inflammatory drugs and stress, in addition to the genetic susceptibility of the patient, represent a minor influ...

Proteome analysis of clustered helicobacter pylori strains

Genomic-methylation typing method, based on strains’ Restriction/Modification systems, confirmed the genetic variability of Helicobacter pylori. According to this, strains isolated from patients of the same family, or from the same geographic region, cluster together. The analysis of proteome’s variability of these clusters has been a missing topic. We applied the Minimum-Common-Restriction-Modification (MCRM) ...

Characterization of new human gastric epithelial cell lines

The lack of a cellular model which correctly mimics the natural niche of the pathogen Helicobacter pylori is still limitative for the study of this infection. Aiming to overcome this limitation, we have previously isolated clones of a subpopulation of the widely used heterogenic NCI-N87 (ATCC CRL-5822) gastric cell line1, those presenting typical epithelial markers and a progenitor-like phenotype (simultaneous ...

Ulcerogenic profile of helicobacter pylori pediatric strains: a contribution to...

Hydrodynamical and biogeochemical modeling study of Sado estuary

The Sado estuary is located in the surrounding area of an important Portuguese natural park (Arrábida Natural Park) near the city of Setúbal. The low freshwater discharge from Sado River, the mesotidal tide along the estuary and the strong currents observed turn the estuary a well‐mixed system, controlling the estuarine residence time, and therefore determining the patterns of conservative and nonconservative t...

DNA encapsulation by biocompatible catanionic vesicles

The encapsulation of DNA by catanionic vesicles has been investigated; the vesicles are composed of one cationic surfactant, in excess, and one anionic. Since cationic systems are often toxic, we introduced a novel divalent cationic amino-acid-based amphiphile, which may enhance transfection and appears to be nontoxic, in our catanionic vesicle mixtures. The cationic amphiphile is arginine-N-lauroyl amide dihyd...

The association of DNA and stable catanionic amino acid-based vesicles

Cationic surfactants associate strongly to DNA and compact but are often toxic. The interaction of some novel cationic amino acid-based surfactants, which may enhance transfection and appear to be nontoxic, is described. A cationic arginine-based surfactant, ALA, gives in combination with anionic surfactants spontaneously stable vesicles, and special attention is given to the association of these catanionic ves...