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Comparison of Fluorescence, 31P NMR, and 7Li NMR Spectroscopic Methods for Inve...
Amari, Louis; Layden, Brian; Rong, Qinfen; Geraldes, Carlos F. G. C.; Freitas, Duarte Mota deThe biochemical action of lithium in the treatment of manic-depressive illness is still unknown. One hypothesis is that Li+ competes for Mg2+-binding sites in biomolecules. We report here our studies on metal ion competition by three distinct methods: fluorescence, 31P NMR, and 7Li NMR spectroscopy, using ATP as a model ligand. By fluorescence spectroscopy, we used the dye, furaptra, by measuring the increases ...
Lithium-7 NMR relaxation study of lithium binding in human erythrocytes
Rong, Qinfen; Espanol, Maryceline; Freitas, Duarte Mota de; Geraldes, Carlos F. G. C.We used 7Li NMR spin-lattice (TI) and spin-spin (Tz) relaxation time measurements to investigate the binding of Li+ in human red blood cell (RBC) suspensions. In RBCs containing 1.4 mM Li+, the intracellular 7Li NMR T2 relaxation value (0.30 f 0.03 s) was much smaller than the corresponding TI value (6.0 f 0.1 s), yielding a ratio of TI to T2 of 20. For 1.5 mM LiCl solutions whose viscosities were adjusted to 5...
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