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Ultrastructural study of in vivo produced ovine embryos: characterization of se...
Bettencourt, Elisa Maria Varela; Bettencourt, Carlos Manuel Varela; Ferreira, Paulo; Romão, Ricardo Jorge; Rocha, António; Sousa, MárioThe ultrastructure of in vivo produced ovine embryos, at the morula, early blastocyst and late blastocyst stages, was evaluated using transmission electron microscopy. In vivo produced embryos (n=14) were obtained from Portuguese Black Merino ewes, superovulated during Spring. Embryos were recovered by abdominal laparotomy, under general anesthesia, on day 6 or 7 after sponge removal. Embryos were processed for...
Cryopreservation of ovine in vitro produced embryos using centrifugation and cy...
Romão, Ricardo Jorge; Marques, Carla; Batista, Maria; Barbas, João; Horta, António; Carolino, Nuno; Bettencourt, Elisa; Pereira, RosaThe cryosurvival of ovine in vitro produced embryos are still low, thus preventing its routine transfer. Attempts have been made to override this problem by decreasing embryos lipid content or protecting their structure with cytoskeletal stabilizers. In this study we used embryo mechanical delipidation through centrifugation in the presence or absence of cytocalasin D testing its effect on embryo quality and cr...
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