Tese de mestrado, Arte, Património e Teoria do Restauro, Universidade de Lisboa, Faculdade de Letras, 2010 ; Resumo disponível em português e inglês
O orçamento público em saúde era da ordem de 53 bilhões de Reais em 2003. Dentro desse orçamento, qual é a parcela referente ao tratamento de vítimas da violência? Devido às limitações dos dados disponíveis não é possível calcular diretamente essa parcela. Neste artigo propomos uma metodologia para estimar o custo da violência para o sistema público de saúde que utiliza informações do Sistema Único de Saúde, do...
O presente documento foi realizado no âmbito da Psicologia do Desporto e tem como objectivo evidenciar alguns dos factores, positivos e negativos, que maior influência exercem sobre as atletas da Liga Portuguesa de Basquetebol. Neste trabalho participaram sete jogadoras da Liga Feminina de Basquetebol, participantes do campeonato de 2003/2004. No total, foram entrevistadas três atletas da equipa do Barreiro, d...
Abstract: The chemosensory system of bacteria controls their motility and behaviour in different environments. In the present study, we report the identification of the first chemotaxis operon in Desulfovibrio gigas. Amino acid sequence analysis revealed seven coding regions for polypeptides with a high similarity to chemotaxis proteins from other organisms. D. gigas chemotaxis operon has a similar genetic orga...
Abstract: Although classified as anaerobic, Desulfovibrio gigas contains a functional canonical membrane respiratory chain, including a cytochrome bd quinol oxidase as its terminal element. In the present study, we report the identification of the operon cydAB encoding the two subunits of cytochrome bd from this bacterium. Two hypothetical promoter regions and sequences resembling transcriptional regulators-bin...
Abstract: Desulfovibrio gigas flavodiiron protein (FDP), rubredoxin: oxygen oxidoreductase (ROO), was proposed to be the terminal oxidase of a soluble electron transfer chain coupling NADH oxidation to oxygen reduction. However, several members from the FDP family, to which ROO belongs, revealed nitric oxide (NO) reductase activity. Therefore, the protection afforded by ROO against the cytotoxic effects of NO w...
Abstract: The interaction and electron transfer (ET) between rubredoxin (Rd) and rubredoxin: oxygen oxidoreductase (ROO) from Desulfovibrio gigas is studied by molecular modelling techniques. Experimental kinetic assays using recombinant proteins show that the Rd reoxidation by ROO displays a bell-shaped dependence on ionic strength, suggesting a non-trivial electrostatic dependence of the interaction between t...
Abstract: In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from ...
Abstract: Aims: In order to evaluate differences between chickpea rhizobial populations from three geographical areas in southern Portugal (Beja, Elvas and Evora), isolates from the three regions were obtained and analysed. Methods and Results: The genetic characterization of the isolates was done by plasmid profiles and restriction analysis of the nif H gene. Symbiotic efficiency of the isolates was also deter...
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