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GABA release by basket cells onto Purkinje cells, in rat cerebellar slices, is ...
Donato, Roberta; Rodrigues, Ricardo J.; Takahashi, Michiko; Tsai, Ming Chi; Soto, David; Miyagi, Kana; Villafuertes, Rosa Gomez; Cunha, Rodrigo A.In many brain regions, Ca2+ influx through presynaptic P2X receptors influences GABA release from interneurones. In patch-clamp recordings of Purkinje cells (PCs) in rat cerebellar slices, broad spectrum P2 receptor antagonists, PPADS (30 [mu]M) or suramin (12 [mu]M), result in a decreased amplitude and increased failure rate of minimal evoked GABAergic synaptic currents from basket cells. The effect is mimicke...
Modification upon aging of the density of presynaptic modulation systems in the...
Canas, Paula M.; Duarte, João M. N.; Rodrigues, Ricardo J.; Köfalvi, Attila; Cunha, Rodrigo A.Different presynaptic neuromodulation systems have been explored as possible targets to manage neurodegenerative diseases. However, most studies used young adult animals whereas neurodegenerative diseases are prevalent in the elderly. Thus, we now explored by Western blot analysis how the density of different presynaptic markers and receptors changes with aging in rat hippocampal synaptosomes (purified nerve te...
Modification of adenosine modulation of acetylcholine release in the hippocampu...
Rodrigues, Ricardo J.; Canas, Paula M.; Lopes, Luísa V.; Oliveira, Catarina R.; Cunha, Rodrigo A.Adenosine is a neuromodulator acting through inhibitory A1 receptors (A1Rs) and facilitatory A2ARs. Since A2AR antagonists attenuate memory deficits in aged animals and memory deficits might involve a decreased cholinergic function, we investigated how aging affects the density and function of adenosine receptors in rat hippocampal cholinergic terminals. In young adult (2 months) rats, 64 and 36% of cholinergic...
Differential glutamate-dependent and glutamate-independent adenosine A1 recepto...
Borycz, Janusz; Pereira, M. Fátima; Melani, Alessia; Rodrigues, Ricardo J.; Köfalvi, Attila; Panlilio, Leigh; Pedata, Felicita; Goldberg, Steven R.Adenosine and dopamine are two important modulators of glutamatergic neurotransmission in the striatum. However, conflicting reports exist about the role of adenosine and adenosine receptors in the modulation of striatal dopamine release. It has been previously suggested that adenosine A1 receptors localized in glutamatergic nerve terminals indirectly modulate dopamine release, by their ability to modulate glut...
Trkb receptors modulation of glutamate release is limited to a subset of nerve ...
Pereira, Daniela B.; Rebola, Nelson; Rodrigues, Ricardo J.; Cunha, Rodrigo A.; Carvalho, Arsélio P.; Duarte, Carlos B.Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to is...
Saccharomyces cerevisiae Hog1 protein phosphorylation upon exposure to bacteria...
Marques, Joana M.; Rodrigues, Ricardo J.; Sant'Ana, Augusto C. de Magalhães; Gonçalves, TeresaThe yeast Hog1 protein is both functionally and structurally similar to the mammalian p38, belonging to the same family of mitogen-activated protein (MAP) kinases and responding to extracellular changes in osmolarity. Since p38 mediates lipopolysaccharide (LPS) effects in mammalian cells, we now tested the responsiveness of Hog1 upon exposure of the yeast Saccharomyces cerevisiae to bacterial LPS. In the presen...
Co-localization and functional interaction between adenosine A2A and metabotrop...
Rodrigues, Ricardo J.; Alfaro, Tiago M.; Rebola, Nelson; Oliveira, Catarina R.; Cunha, Rodrigo A.The anti-Parkinsonian effect of glutamate metabotropic group 5 (mGluR5) and adenosine A2A receptor antagonists is believed to result from their ability to postsynaptically control the responsiveness of the indirect pathway that is hyperfunctioning in Parkinson's disease. mGluR5 and A2A antagonists are also neuroprotective in brain injury models involving glutamate excitotoxicity. Thus, we hypothesized that the ...
Dual presynaptic control by ATP of glutamate release via facilitatory P2X1, P2X...
Rodrigues, Ricardo J.; Almeida, Teresa; Richardson, Peter J.; Oliveira, Catarina R.; Cunha, Rodrigo A.ATP is released in a vesicular manner from nerve terminals mainly at higher stimulation frequencies. There is a robust expression of ATP (P2) receptors in the brain, but their role is primarily unknown. We report that ATP analogs biphasically modulate the evoked release of glutamate from purified nerve terminals of the rat hippocampus, the facilitation being mediated by P2X1, P2X2/3, and P2X3 [antagonized by 8-...
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