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Lavado traqueobrônquico por via nasotraqueal como metodologia de colheita de cé...
Marcondes,J.S.; Martins,M.T.A.; Silva,A.A.; Rodrigues,M.M.P.; Ferreira,D.O.L.; Amorim,R.L.; Dias,A.; Gonçalves,R.C.Os estudos das secreções traqueobrônquicas são amplamente utilizados nas pesquisas de doenças pulmonares nas diversas espécies animais, inclusive no homem. Os objetivos desta pesquisa foram a viabilização da técnica de colheita de lavado traqueobrônquico na espécie ovina e o estudo da relação clínico-citológica do lavado de ovinos portadores de broncopneumonia e sadios. Foram utilizados 33 ovinos, 18 sadios e 1...
Plasma concentrations and placental immunostaining of interleukin-10 and tumorn...
Sinzato,Y.K.; Damasceno,D.C.; Laufer-Amorim,R.; Rodrigues,M.M.P.; Oshiiwa,M.; Taylor,K.N.; Rudge,M.V.C.Interleukin-10 (IL-10) appears to be the key cytokine for the maintenance of pregnancy and inhibits the secretion of inflammatory cytokines such as tumor necrosis factor-α (TNF-α). However, there are no studies evaluating the profile of these cytokines in diabetic rat models. Thus, our aim was to analyze IL-10 and TNF-α immunostaining in placental tissue and their respective concentrations in mat...
Immunodominance: a new hypothesis to explain parasite escape and host/parasite ...
Rodrigues,M.M.; Alencar,B.C.G. de; Claser,C.; Tzelepis,F.Intense immune responses are observed during human or experimental infection with the digenetic protozoan parasite Trypanosoma cruzi. The reasons why such immune responses are unable to completely eliminate the parasites are unknown. The survival of the parasite leads to a parasite-host equilibrium found during the chronic phase of chagasic infection in most individuals. Parasite persistence is recognized as th...
Effects of age and mannanoligosaccharides supplementation on production of vola...
Guedes, Cristina; Mourão, José Luís; Silva, Severiano; Gomes, Maria José; Rodrigues, M.M.; Pinheiro, VictorThis trial studied the effect of including mannanoligosaccharides (MOS, Bio-Mos®, Alltech Inc., USA) in the diet on the caecal volatile fatty acids (VFA) and pH of rabbits from 34 to 90 days of age. Three experimental diets were compared: control diet, zinc bacitracin (ZnBac) diet (control diet with 0.1 g ZnBac/kg feed) and MOS diet (control diet with 2.0 g MOS/kg feed). Rabbits were slaughtered at 34, 48, 69 a...
El uso de hoja de olivo tratada con hongos (Gannoderma applanatum) en dietas pa...
Pinheiro, V.M; Ribeiro, L; Outor-Monteiro, D; Rodrigues, M.M; Bezerra, R; Dias, A; Mourão, J.LEn un experimento se ha estudiado el efecto de la incorporación de hoja de olivo sola o tratada con hongos (Gannoderma applanatum) en dietas para conejos en final de crecimiento. Se han utilizado 3 dietas, una dieta base (HO0) a la cual se ha añadido un 5% de hojas de olivo no tratadas (HO5) o incubadas con hongos (HO5H). Se han controlado la eficacia de crecimiento (entre los 52 y 73 d), la digestibilidad (62 ...
The non-palindromic adaptor-PCR method for the identification of the T-cell rec...
Hiyane,M.I.; Boscardin,S.B.; Rodrigues,M.M.Cloning of the T-cell receptor genes is a critical step when generating T-cell receptor transgenic mice. Because T-cell receptor molecules are clonotypical, isolation of their genes requires reverse transcriptase-assisted PCR using primers specific for each different Valpha or Vß genes or by the screening of cDNA libraries generated from RNA obtained from each individual T-cell clone. Although feasible, these a...
Trans-sialidase delivered as a naked DNA vaccine elicits an immunological respo...
Costa,F.; Pereira-Chioccola,V.L.; Ribeirão,M.; Schenkman,S.; Rodrigues,M.M.Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, does not synthesize sialic acid, but expresses a trans-sialidase (TS) that catalyzes the transfer of sialic acid from host glycoconjugates to the parasite surface. Here, we review studies that characterize the immune response to the catalytic domain of the enzyme in humans during Chagas' disease or in mice following immunization with the TS ...
Malaria vaccine: roadblocks and possible solutions
Soares,I.S.; Rodrigues,M.M.Malaria remains the most prevalent and devastating parasitic disease worldwide. Vaccination is considered to be an approach that will complement other strategies for prevention and control of the disease in the future. In the last 10 years, intense studies aimed at the development of a malaria vaccine have provided important knowledge of the nature of the host immunological mechanisms of protection and their re...
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