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Cryopreservation of cell laden natural origin hydrogels for cartilage regenerat...

The time span needed for obtaining a functional cartilage substitute using tissue engineering strategies, together with the need for specific patient oriented constructs has stimulated the growing interest for developing “off-the shelf” products. One way to deliver such products is based on long-term storage processes, such as cryopreservation, that will provide the clinical substitute available as needed and c...

Understanding the role of growth factors in modulating stem cell tenogenesis

Current treatments for tendon injuries often fail to fully restore joint biomechanics leading to the recurrence of symptoms, and thus resulting in a significant health problem with a relevant social impact worldwide. Cell-based approaches involving the use of stem cells might enable tailoring a successful tendon regeneration outcome. As growth factors (GFs) powerfully regulate the cell biological response, thei...

Magnetic nanoparticles potential for stem cell functionalization

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; Stem cells have the capacity to migrate within tissues to damaged areas. The ability to remotely monitor and manipulate cells encouraging their precise positioning to desired sites for tissue regeneration would have a potential impact in tissue engineering and regenerative medicine. Cell monitoring and cell localiz...

Magnetic-responsive hydrogels for cartilage tissue engineering

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013) ; The use of magnetic nanoparticles (MNPs) has been explored as an alternative approach to overcome current limitations of regenerative medicine strategies. Cell engineering approaches where MNPs are incorporated within three-dimensional constructs, such as scaffolds or hydrogels may constitute a novel and attractive...

Tenogenic potential of human stem cells from the amniotic fluid and adipose tissue

Tendons are highly prone to injury and the intrinsic hypocellularity and hypovascularity make their natural healing extremely slow and inefficient when severed damaged. Surgical repair with grafts is common but unsuccessful in a long term basis. The development of tissue engineering strategies based on stem cells explores a natural endogenous system of regeneration with potential for tendon application. We prop...

Induction of tenogenic differentiation in human adipose stem cells by manipulat...

The limited ability of tendon to self-repair and the limitation of treatment regimes have hastened the motivation to develop cell-based strategies for tendon repair. Growth factors (GFs) such as EGF, FGF, PDGF and TGF-b participate in tendon formation, ECM synthesis or healing, and may assist tenogenic differentiation. Thus, this work aims to establish culturing conditions that induce tenogenic differentiation ...

Engineering tendon and ligament tissues : present developments towards successf...

Musculoskeletal diseases are one of the leading causes of disability worldwide. Among them, tendon and ligament injuries represent an important aspect to consider in both athletes and active working people. Tendon and ligament damage is an important cause of joint instability, and progresses into early onset of osteoarthritis, pain, disability and eventually the need for joint replacement surgery. The social an...

Bilayered constructs aimed at osteochondral strategies : the influence of media...

injuries and aging associated diseases that affect joints. This study reports the development of a bilayered scaffold, which consists of both bone and cartilage regions. On the other hand, amniotic fluid-derived stem cells (AFSCs) could be differentiated into either osteogenic or chondrogenic cells, respectively. In this study we have developed a bilayered scaffolding system, which includes a starch/polycaprola...

Synergistic effect of scaffold composition and dynamic culturing environment in...

Bone extracellular matrix (ECM) is composed of mineralized collagen fibrils which support biological apatite nucleation that participates in bone outstanding properties. Understanding and mimicking bone morphological and physiological parameters at a biological scale is a major challenge in tissue engineering scaffolding. Using emergent (nano)technologies scaffold designing may be critically improved, enabling ...

Cell encapsulation system using beads and fibres from natural origin polymers f...