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Chemical composition and antioxidant activity of sulphated polysaccharides extr...

Sulphated polysaccharides (SP) were extracted from Fucus vesiculosus seaweed by using two different hydrothermal processes: microwave-assisted extraction (MAE) and autohydrolysis (AH). The extraction yields, chemical composition, and antioxidant activity of the polysaccharides extracted were determined and compared. Although both processes afforded SP with similar yields (18.2 mass % and 16.5 mass %, for MAE an...

Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus...

The extraction of sulfated polysaccharides (fucoidan) by autohydrolysis (AH) of brown seaweed Fucus vesiculosus was studied. Experimental assays were performed under different conditions of temperature (160 to 200°C) and reaction time (10 to 30 min) according to a 22 central composite design, and the conditions able to maximize the fucoidan yield were selected. The alga degradation and the total sugar yield in ...

Biorefinery valorization of autohydrolysis wheat straw hemicellulose to be appl...

The aims of this study were the extraction of hemicellulose from wheat straw (WS) and its utilization in the reinforcement of a κ-carrageenan/locust bean gum (κ-car/LBG) polymeric blend films (PBFs). WS hemicellulose extraction was performed under autohydrolysis process and hemicellulose extracted (HE) under optimum condition was used in PBFs. PBFs were prepared varying different proportions of HE into the κ-ca...

Hydrothermal processing, as an alternative for upgrading agriculture residues a...

The concept of a biorefinery that integrates processes and technologies for biomass conversion demands efficient utilization of all components. Hydrothermal processing is a potential clean technology to convert raw materials such as lignocellulosic materials and aquatic biomass into bioenergy and high added-value chemicals. In this technology, water at high temperatures and pressures is applied for hydrolysis, ...

Growth of fungal strains on coffee industry residues with removal of polyphenol...

Coffee silverskin (CS) and spent coffee grounds (SCG) are abundant residues generated by the coffee industry, but up till now they are practically unutilized being discharged to the environment or burned for elimination. In the present study, the ability of seven different fungal strains from the genera Aspergillus, Mucor, Penicillium, and Neurospora, to grow and release phenolic compounds from these residues u...

Development of fermentation process for fungal fucoidanases production

Dissertação de mestrado em Chemical and Biological Engineering ; This thesis is focused in the development of an integral bioprocess for fungal fucoidanases production, in order to develop new strategies of fermentative conditions and parameters. In the recent years, the interest in the research field of fucoidans and fucoidanases has strongly increased due to the high potential in medical exploitation of fuco...

Microwave-assisted extraction of sulfated polysaccharides(fucoidan)from brown s...

Sulfated polysaccharides (fucoidan) were recovered from brown seaweed Fucus vesiculosus by microwave-assisted extraction (MAE). Different conditions of pressure (30 to 120 psi), extraction time (1 to 31 min), and alga/water ratio (1/25 to 5/25 g ml-1) were evaluated during this process aiming to establish a condition to maximize the extraction results. The alga degradation (%), total sugar yield (%), and SO3 co...

Extraction and characterization of sulfated fucans from brown seaweed fucus ves...

Fucoidan-degrading fungal strains : screening, morphometric evaluation, and inf...

Ten different fungal strains from the genus Aspergillus, Penicillium, and Mucor were screened for fucoidan hydrolyzing ability aiming to find microorganisms able to produce sulfated fucan-degrading enzymes. Screening was carried out by measuring the strains kinetic and morphometric behavior over plate assays using Laminaria japonica fucoidan as only carbon source, testing three nitrogen sources (urea, peptone, ...

Adaptation of dinitrosalicylic acid method to microtiter plates

A microtiter plate adaptation of the well-known dinitrosalicylic acid (DNS) colorimetric method, for measurement of reducing sugars, is described. This method allows a substantial volume reduction of the reagents and a rapid analysis of a large number of samples, having an economic cost benefit and a positive impact on the environment. Therefore, it is well suited as a high-throughput technique for reducing sug...