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Semi-solid-state fermentation : a promising alternative for neomycin production...
Machado, Isabel; Teixeira, J. A.; Rodríguez Couto, S.The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum value of 13,903 μ...
Application of response surface methodological approach to optimise Reactive Bl...
Roriz, Margarida S.; Osma, Johann F.; Teixeira, J. A.; Rodríguez Couto, S.Response surface methodology (RSM) was applied to optimise the decolouration of the diazo dye Reactive Black 5 (RB5) by crude laccase from the white-rot fungus Trametes pubescens. The presence of the redox mediator 1-hydroxybenzotriazole (HBT) greatly improved the decolouration levels of RB5 by crude laccase from T. pubescens. Central composite design (CCD) using RSM with three variables namely redox mediator (...
Enzymatic synthesis of Tinuvin
Schroeder, M.; Pereira, Luciana; Rodríguez Couto, S.; Erlacher, Angelika; Schoening, K.-U.; Paulo, Artur Cavaco; Guebitz, G. M.Coupling of 3-(3-tert-butyl-4-hydroxyphenyl) propionic acid methylester to 1H-benzotriazole using a laccase from Trametes hirsuta was studied. The potentially resulting coupling product Tinuvin 1130 is an important UV-absorber used in polymer based materials. Oxidation of the phenol by the laccase led to homomolecular coupling reactions while the laccase did not attack 1H-benzotriazole. Due to the homomolecular...
Laccase activity from the fungus trametes hirsuta using an air-lift bioreactor
Rodríguez Couto, S.; Rodríguez, A.; Paterson, R. R. M.; Lima, Nelson; Teixeira, J. A.Aim: To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB). Methods and Results: Trametes hirsuta was grown in a 6-l ALB. A fed-batch strategy with glycerol as an addition resulted in maximum laccase activity of 19 400 U l)1, which was the highest reported from the fungus. Conclusion: The ALB configuration with additional glycerol resulted in high...
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