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Laccase recovery with aqueous two-phase systems : enzyme partitioning and stabi...

In this work, the potential of several aqueoustwo-phase systems (ATPS) for laccaserecovery was evaluated. For this purpose, different types of ATPS were prepared and the partition coefficient of pure commercial laccase was determined in each one, at 23 °C. A total of 21 ATPS were investigated: 12 composed by a polymer and a salt and 9 composed by two different polymers. For polymer-salt ATPS, different composit...

(Liquid + liquid) equilibria of polymer-salt aqueous two-phase systems for lacc...

Aqueous two-phase systems (ATPS) are recognized as very suitable techniques for the recovery of target solutes in biological applications. Three new phase diagrams of (UCON 50-HB-5100 + potassium citrate + water), (UCON 50-HB-5100 + sodium formate + water), and (UCON 50-HB-5100 + potassium formate + water) systems were measured at 23 C. The binodal curves were successfully described using the empirical equation...

Liquid-liquid equilibria of UCON + (sodium or potassium) phosphate salt aqueous...

Six phase diagrams for UCON−phosphate salt aqueous two-phase systems (ATPSs), at 23 °C, are presented. The ATPSs were obtained combining UCON (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide) and a phosphate salt (KH2PO4, K2HPO4, potassium phosphate buffer pH = 7, NaH2PO4, Na2HPO4, and sodium phosphate buffer pH = 7). Among the salts used, Na2HPO4 proved to be the most effective in ATPS form...

Gibbs free energy of transfer of a methylene group on {UCON + (sodium or potass...

The Gibbs free energy of transfer of a suitable hydrophobic probe can be regarded as a measure of the relative hydrophobicity of the different phases. The methylene group (CH2) can be considered hydrophobic, and thus be a suitable probe for hydrophobicity. In this work, the partition coefficients of a series of five dinitrophenylated-amino acids were experimentally determined, at 23 °C, in three different tie-l...

Solute partitioning in polymer–salt ATPS : the collander equation

The partition coefficients for several solutes (five nitrophenylated-monosaccharides and four proteins) were experimentally determined, at 23 ◦C, in three different tie-lines of two polymer–salt aqueous twophase systems (ATPS): UCON-K2HPO4 and UCON-NaH2PO4. These partition coefficients together with others obtained from the literature for five dinitrophenylated-amino acids were used to investigate the suitabili...

Relative hydrophobicity of (PEG or Ucon)-salt ATPSs

Aqueous Two-Phase Systems (ATPSs) are biphasic systems composed mainly by water. ATPSs are obtained upon mixing of two aqueous solutions of certain polymers or a polymer and a salt (above certain critical conditions, e.g. concentration, temperature). These systems are commonly indicated for the extraction of biomolecules. In this work, the partition coefficients for a series of five dinitrophenylated amino-acid...

ΔG(CH2) in PEG-Salt and Ucon-Salt Aqueous Two-Phase Systems

Three new phase diagrams for polymer−salt aqueous two-phase systems (ATPS) at 23 °C are presented. Then, partition coefficients for a series of five dinitrophenylated amino acids were obtained experimentally in five polymer−salt ATPS. The ATPS were obtained combining a polymer, polyethylene glycol (PEG) or Ucon (a random copolymer of 50 % ethylene oxide and 50 % propylene oxide), and a salt (Na2SO4, (NH4)2SO4, ...

Physicochemical characterization of the PEG8000-Na2SO4 aqueous two-phase system

The polyethylene glycol-sodium sulfate aqueous two-phase system has been characterized at 23 °C. Tielines for the phase diagram were obtained experimentally. Phases in equilibrium were characterized by means of the solvatochromic parameters π*, α, and β, which provide a measurement of the polarity/polarizability and the H-bond donor and acceptor abilities, respectively. The ability of the phases to participate ...

Biomolecules partition in aqueous (PEG + Na2SO4) two-phase system

Prevalence of blood parasites in Tyrannidae (flycatchers) in the Eastern plains...

Blood samples from 159 birds of the New-world family Tyrannidae (the flycatchers) from the eastern plains of Colombia, were examined for haematozoa parasites, in 1999-2000. Haematozoa were detected in six of 20 species. The overall prevalence was 10.1%. The most common parasites detected were microfilariae, followed by Trypanosoma and Plasmodium. The highest prevalence (9.6%) was found in the Ochre-bellied Flyc...