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A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Genomic organization and dynamics of repetitive DNA sequences in representative...
Alves, Sofia; Ribeiro, Teresa; Inácio, Vera; Rocheta, Margarida; Morais-Cecílio, LeonorOaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand the relationship between these members of this family, a deep knowledge of their genome composition and organization is needed. In this work, we have isolated and characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involvi...
Microarray-based uncovering reference genes for quantitative real time PCR in g...
Coito, João L.; Rocheta, Margarida; Carvalho, Luísa; Amâncio, SaraResearch Article ; Background: Quantitative real time polymerase chain reaction is becoming the primary tool for detecting mRNA and transcription data analysis as it shows to have advantages over other more commonly used techniques. Nevertheless, it also presents a few shortcomings, with the most import being the need for data normalisation, usually with a reference gene. Therefore the choice of the reference ...
Polyploidization as a retraction force in plant genome evolution: sequence rear...
Silva, Manuela; Viegas, Wanda; Bento, Miguel; Pereira, H. Sofia; Rocheta, Margarida; Gustafson, PerryBackground. Polyploidization is a major evolutionary process in plants where hybridization and chromosome doubling induce enormous genomic stress and can generate genetic and epigenetic modifications. However, proper evaluation of DNA sequence restructuring events and the precise characterization of sequences involved are still sparse. Methodology/Principal Findings. Inter Retrotransposons Amplified Polymorphis...
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