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Polyphenoloxidase activity of minimally processed "Jonagored" apples (Malus dom...

Rocha, A. M. C. N.; Morais, A. M. M. B. de

The influence of three chemical dips using ascorbic acid (AA), citric acid (CA) and calcium chloride (CC) on the polyphenoloxidase (PPO) activity and on the total phenolic content of minimally processed (MP) apple (Malus domestica, cv. Jonagored) during cold storage was evaluated and a potential relationship with enzymatic browning was investigated. An ascorbic acid dip (42.6 mM) of 5 min duration was the most ...


Modified atmosphere package for apple ‘Bravo de Esmolfe’

Rocha, A.M.C.N.; Barreiro, M.G.; Morais, A.M.M.B.

Apple Bravo de Esmolfe original from the parish of Esmolfe is cultivated in specific regions of Portugal. This cultivar of medium to low production requires very low temperatures, but it is extremely sensitive to frost. The aim of this work was to evaluate the influence of modified atmosphere on the quality of apple (cv. Bravo de Esmolfe) during cold storage. Apples packed in modified atmosphere lost less w...


Shelf life of minimally processed apple (cv. Jonagored) determined by colour ch...

Rocha, A. M. C. N.; Morais, A. M. M. B.

Physical, chemical and sensory changes of cut apple (cv. Jonagored) stored in the dark at 4 °C were evaluated. Colour was found to be the critical parameter for this product. Apple cubes underwent severe surface browning primarily during the initial days of storage. The shelf-life of cut apple was therefore very limited, to three days maximum. Sensory analyses and objective quality evaluations of cut apple wer...


Characterization of polyphenoloxidase (PPO) extracted from "Jonagored" apple

Rocha, A.M.C.N.; Morais, A. M. M. B.

Polyphenoloxidase (PPO) was extracted from apple (cv. Jonagored) with addition of 2% PVP and 0.25% Triton X100 to the extraction buffer containing phenolic adsorbents. Experiments were performed to evaluate the affinity and specificity towards several substrates. ‘Jonagored’ apple PPO was found to have higher specificity (lower Km) towards L-dopa, 4-methylcatechol and (+) catechin than other phenols tested, but...


Influence of controlled atmosphere storage on polyphenoloxidase activity in rel...

Rocha, A. M. C. N.; Morais, M. M. B.

The effects of the storage atmosphere composition (2% O2 + 4% CO2; 2% O2 + 8% CO2; 2% O2 + 12% CO2) on polyphenoloxidase activity and phenolic content of the ‘Jonagored’ apple variety during cold storage was evaluated, and the relationship with enzymatic browning investigated. Controlled atmosphere storage inhibited the polyphenoloxidase (PPO) activity of apple cubes during storage. It seems that the higher the...




Influence of chemical treatment on quality of cut apple (cv. jonagored)

Rocha, A.M.C.N.; Brochado, C.M.; Morais, A.M.M.B

The quality of a new variety of apple, ‘Jonagored’, after peeling and cutting was evaluated during ten days of storage in air at 4C. The objective was to evaluate the shelf-life of apple cubes by detecting the limiting parameter and to test the influence of different chemical dips on the cut apple quality with the aim of extending shelf-life. Color, i.e. browning at the cut surface, was found to be the critical...



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Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia